The role of the ELAV homologue EXC-7 in the development of the Caenorhabditis elegans excretory canals

Masaki Fujita, Dana Hawkinson, Kevin V. King, David H. Hall, Hiroshi Sakamoto, Matthew Buechner

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The exc mutations of Caenorhabditis elegans alter the position and shape of the apical cytoskeleton in polarized epithelial cells. Mutants in exc-7 form small cysts throughout the tubular excretory canals that regulate organismal osmolarity. We have cloned the exc-7 gene, the closest nematode homologue to the neural RNA-binding protein ELAV. EXC-7 is expressed in the canal for a short time midway through embryogenesis. Cysts in exc-7 mutants do not develop until several hours later, beginning at the time of hatching. We find that the first larval period is when the canal completes the majority of its outgrowth, and adds new apical cytoskeleton at a rapid rate. Ultrastructural studies show that exc-7 mutant defects resemble loss of βH-spectrin (encoded by sma-1) at the distal ends of the excretory canals. In addition, exc-7 mutants exhibit synergistic excretory canal defects with mutations in sma-1, and EXC-7 binds sma-1 mRNA. These data imply that EXC-7 protein may affect expression of sma-1 and other genes to effect proper development of the excretory canals.

Original languageEnglish (US)
Pages (from-to)290-301
Number of pages12
JournalDevelopmental Biology
Volume256
Issue number2
DOIs
StatePublished - Apr 15 2003

Fingerprint

Caenorhabditis elegans
Cytoskeleton
Cysts
Spectrin
Mutation
RNA-Binding Proteins
Osmolar Concentration
Genes
Embryonic Development
Epithelial Cells
Messenger RNA
Proteins

Keywords

  • Apical cytoskeleton
  • ELAV
  • Epithelial polarity
  • Excretory canals
  • RNA-binding
  • Spectrin

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The role of the ELAV homologue EXC-7 in the development of the Caenorhabditis elegans excretory canals. / Fujita, Masaki; Hawkinson, Dana; King, Kevin V.; Hall, David H.; Sakamoto, Hiroshi; Buechner, Matthew.

In: Developmental Biology, Vol. 256, No. 2, 15.04.2003, p. 290-301.

Research output: Contribution to journalArticle

Fujita, Masaki ; Hawkinson, Dana ; King, Kevin V. ; Hall, David H. ; Sakamoto, Hiroshi ; Buechner, Matthew. / The role of the ELAV homologue EXC-7 in the development of the Caenorhabditis elegans excretory canals. In: Developmental Biology. 2003 ; Vol. 256, No. 2. pp. 290-301.
@article{0b4dfc55c9634803833499071968b817,
title = "The role of the ELAV homologue EXC-7 in the development of the Caenorhabditis elegans excretory canals",
abstract = "The exc mutations of Caenorhabditis elegans alter the position and shape of the apical cytoskeleton in polarized epithelial cells. Mutants in exc-7 form small cysts throughout the tubular excretory canals that regulate organismal osmolarity. We have cloned the exc-7 gene, the closest nematode homologue to the neural RNA-binding protein ELAV. EXC-7 is expressed in the canal for a short time midway through embryogenesis. Cysts in exc-7 mutants do not develop until several hours later, beginning at the time of hatching. We find that the first larval period is when the canal completes the majority of its outgrowth, and adds new apical cytoskeleton at a rapid rate. Ultrastructural studies show that exc-7 mutant defects resemble loss of βH-spectrin (encoded by sma-1) at the distal ends of the excretory canals. In addition, exc-7 mutants exhibit synergistic excretory canal defects with mutations in sma-1, and EXC-7 binds sma-1 mRNA. These data imply that EXC-7 protein may affect expression of sma-1 and other genes to effect proper development of the excretory canals.",
keywords = "Apical cytoskeleton, ELAV, Epithelial polarity, Excretory canals, RNA-binding, Spectrin",
author = "Masaki Fujita and Dana Hawkinson and King, {Kevin V.} and Hall, {David H.} and Hiroshi Sakamoto and Matthew Buechner",
year = "2003",
month = "4",
day = "15",
doi = "10.1016/S0012-1606(03)00040-X",
language = "English (US)",
volume = "256",
pages = "290--301",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The role of the ELAV homologue EXC-7 in the development of the Caenorhabditis elegans excretory canals

AU - Fujita, Masaki

AU - Hawkinson, Dana

AU - King, Kevin V.

AU - Hall, David H.

AU - Sakamoto, Hiroshi

AU - Buechner, Matthew

PY - 2003/4/15

Y1 - 2003/4/15

N2 - The exc mutations of Caenorhabditis elegans alter the position and shape of the apical cytoskeleton in polarized epithelial cells. Mutants in exc-7 form small cysts throughout the tubular excretory canals that regulate organismal osmolarity. We have cloned the exc-7 gene, the closest nematode homologue to the neural RNA-binding protein ELAV. EXC-7 is expressed in the canal for a short time midway through embryogenesis. Cysts in exc-7 mutants do not develop until several hours later, beginning at the time of hatching. We find that the first larval period is when the canal completes the majority of its outgrowth, and adds new apical cytoskeleton at a rapid rate. Ultrastructural studies show that exc-7 mutant defects resemble loss of βH-spectrin (encoded by sma-1) at the distal ends of the excretory canals. In addition, exc-7 mutants exhibit synergistic excretory canal defects with mutations in sma-1, and EXC-7 binds sma-1 mRNA. These data imply that EXC-7 protein may affect expression of sma-1 and other genes to effect proper development of the excretory canals.

AB - The exc mutations of Caenorhabditis elegans alter the position and shape of the apical cytoskeleton in polarized epithelial cells. Mutants in exc-7 form small cysts throughout the tubular excretory canals that regulate organismal osmolarity. We have cloned the exc-7 gene, the closest nematode homologue to the neural RNA-binding protein ELAV. EXC-7 is expressed in the canal for a short time midway through embryogenesis. Cysts in exc-7 mutants do not develop until several hours later, beginning at the time of hatching. We find that the first larval period is when the canal completes the majority of its outgrowth, and adds new apical cytoskeleton at a rapid rate. Ultrastructural studies show that exc-7 mutant defects resemble loss of βH-spectrin (encoded by sma-1) at the distal ends of the excretory canals. In addition, exc-7 mutants exhibit synergistic excretory canal defects with mutations in sma-1, and EXC-7 binds sma-1 mRNA. These data imply that EXC-7 protein may affect expression of sma-1 and other genes to effect proper development of the excretory canals.

KW - Apical cytoskeleton

KW - ELAV

KW - Epithelial polarity

KW - Excretory canals

KW - RNA-binding

KW - Spectrin

UR - http://www.scopus.com/inward/record.url?scp=0344211463&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344211463&partnerID=8YFLogxK

U2 - 10.1016/S0012-1606(03)00040-X

DO - 10.1016/S0012-1606(03)00040-X

M3 - Article

C2 - 12679103

AN - SCOPUS:0344211463

VL - 256

SP - 290

EP - 301

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -