The role of ribosomal proteins L7 and L12 in polypeptide chain initiation in Escherichia coli

A. H. Lockwood, U. Maitra, N. Brot, H. Weissbach

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Proteins L7 and L12 from the 50 S ribosomal subunit are required for polypeptide chain initiation in Escherichia coli. Ribosomes deficient in these proteins cannot participate in initiation factor IF 2 dependent hydrolysis of guanosine triphosphate (either 'coupled' or 'uncoupled' from initiation); activity can be restored by the addition of either L7 or L12. N Formyl methionyl transfer RNA binding to ribosomes is also impaired in the absence of L7 and L12. The results indicate that L7 or L12 is required for the catalytic function of IF 2 in binding N formyl methionyl transfer RNA to ribosomes. In each reaction tested, L7 and L12 are about equal in efficiency in restoring activity to depleted 50 S subunits. These results are discussed in the light of the current information on the role of these ribosomal proteins in other partial reactions or protein synthesis.

Original languageEnglish (US)
Pages (from-to)1213-1218
Number of pages6
JournalJournal of Biological Chemistry
Volume249
Issue number4
StatePublished - 1974

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L 012
Transfer RNA
Escherichia coli
Ribosomes
Peptide Initiation Factors
Peptides
Ribosomal Proteins
Guanosine Triphosphate
Hydrolysis
Proteins
Prokaryotic Initiation Factor-2
Ribosome Subunits
ribosomal protein L7-L12

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lockwood, A. H., Maitra, U., Brot, N., & Weissbach, H. (1974). The role of ribosomal proteins L7 and L12 in polypeptide chain initiation in Escherichia coli. Journal of Biological Chemistry, 249(4), 1213-1218.

The role of ribosomal proteins L7 and L12 in polypeptide chain initiation in Escherichia coli. / Lockwood, A. H.; Maitra, U.; Brot, N.; Weissbach, H.

In: Journal of Biological Chemistry, Vol. 249, No. 4, 1974, p. 1213-1218.

Research output: Contribution to journalArticle

Lockwood, AH, Maitra, U, Brot, N & Weissbach, H 1974, 'The role of ribosomal proteins L7 and L12 in polypeptide chain initiation in Escherichia coli', Journal of Biological Chemistry, vol. 249, no. 4, pp. 1213-1218.
Lockwood, A. H. ; Maitra, U. ; Brot, N. ; Weissbach, H. / The role of ribosomal proteins L7 and L12 in polypeptide chain initiation in Escherichia coli. In: Journal of Biological Chemistry. 1974 ; Vol. 249, No. 4. pp. 1213-1218.
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