The role of protein kinase C in laminin-mediated neurite outgrowth

Benjamin S. Weeks, Paul J. Wilson, Cathleen C. Heffernan, Ammar Ahmad, Kris Mahadeo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Laminin is a potent stimulator of neurite outgrowth in rat pheochromocytoma (PC12) cells. Here, we investigated the role of protein kinase C (PKC) in the mechanism of laminin-mediated neurite outgrowth in PC12 cells. Phorbol ester activators of PKC have been shown to have divergent effects on laminin-mediated neurite outgrowth. Therefore, we tested the effect of the nonphorbol PKC activator, indolactam V. At 1.0 μM indolactam V inhibited laminin-mediated neurite outgrowth by 85%. Further, the PKC inhibitor H7 blocked the inhibitory effect of indolactam V on laminin-mediated neurite outgrowth. Direct measurement of protein kinase C activity in the soluble (cytosolic) and particulate (membrane) fractions of PC12 cells showed that laminin failed to alter protein kinase C activity. These data demonstrate that PKC activation inhibits laminin-mediated neurite outgrowth and that laminin does not activate PKC in PC12 cells.

Original languageEnglish (US)
Pages (from-to)98-103
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume256
Issue number1
DOIs
StatePublished - Mar 5 1999
Externally publishedYes

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Laminin
Protein Kinase C
PC12 Cells
Neuronal Outgrowth
Protein C Inhibitor
Pheochromocytoma
Phorbol Esters
Protein Kinase Inhibitors
Rats
Chemical activation
Membranes
indolactam V

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The role of protein kinase C in laminin-mediated neurite outgrowth. / Weeks, Benjamin S.; Wilson, Paul J.; Heffernan, Cathleen C.; Ahmad, Ammar; Mahadeo, Kris.

In: Biochemical and Biophysical Research Communications, Vol. 256, No. 1, 05.03.1999, p. 98-103.

Research output: Contribution to journalArticle

Weeks, Benjamin S. ; Wilson, Paul J. ; Heffernan, Cathleen C. ; Ahmad, Ammar ; Mahadeo, Kris. / The role of protein kinase C in laminin-mediated neurite outgrowth. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 256, No. 1. pp. 98-103.
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