The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation

Choul Yong Park, Seung Ha Yang, Roy S. Chuck, Peter L. Gehlbach, Chung Gyu Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. Methods: The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-γ stimulation. Results: RPEs expressed IDO and class II MHC molecules under IFN-γ stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-γ prestimulation. This phenomenon was enhanced by IFN-γ stimulation. The addition of 1-methyl tryptophan (1-MT) into the culture medium successfully reversed RPE immunosuppression. Conclusions: The expression of IDO may in part explain RPE-mediated immune suppression effects.

Original languageEnglish (US)
Pages (from-to)24-31
Number of pages8
JournalOcular Immunology and Inflammation
Volume18
Issue number1
DOIs
StatePublished - Jan 2010

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Dioxygenases
Retinal Pigments
Epithelial Cells
Mixed Lymphocyte Culture Test
Major Histocompatibility Complex
Immunosuppression
B7 Antigens
Reverse Transcriptase Polymerase Chain Reaction
Fluorescent Antibody Technique
Culture Media
Flow Cytometry
Staining and Labeling

Keywords

  • 3-dioxygenase
  • Co-stimulatory molecule
  • Immune modulation
  • Indoleamine 2
  • Major histocompatibility complex
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Ophthalmology
  • Immunology and Allergy

Cite this

The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation. / Park, Choul Yong; Yang, Seung Ha; Chuck, Roy S.; Gehlbach, Peter L.; Park, Chung Gyu.

In: Ocular Immunology and Inflammation, Vol. 18, No. 1, 01.2010, p. 24-31.

Research output: Contribution to journalArticle

Park, Choul Yong ; Yang, Seung Ha ; Chuck, Roy S. ; Gehlbach, Peter L. ; Park, Chung Gyu. / The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation. In: Ocular Immunology and Inflammation. 2010 ; Vol. 18, No. 1. pp. 24-31.
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