The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation

Choul Yong Park; Seung Ha Yang; Roy S. Chuck; Peter L. Gehlbach; Chung Gyu Park

doi:10.3109/09273940903326203

The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation

Choul Yong Park, Seung Ha Yang, Roy S. Chuck, Peter L. Gehlbach, Chung Gyu Park

Ophthalmology & Visual Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Purpose: To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. Methods: The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-γ stimulation. Results: RPEs expressed IDO and class II MHC molecules under IFN-γ stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-γ prestimulation. This phenomenon was enhanced by IFN-γ stimulation. The addition of 1-methyl tryptophan (1-MT) into the culture medium successfully reversed RPE immunosuppression. Conclusions: The expression of IDO may in part explain RPE-mediated immune suppression effects.

Original language	English (US)
Pages (from-to)	24-31
Number of pages	8
Journal	Ocular Immunology and Inflammation
Volume	18
Issue number	1
DOIs	https://doi.org/10.3109/09273940903326203
State	Published - Jan 2010

Keywords

3-dioxygenase
Co-stimulatory molecule
Immune modulation
Indoleamine 2
Major histocompatibility complex
Retinal pigment epithelium

ASJC Scopus subject areas

Immunology and Allergy
Ophthalmology

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10.3109/09273940903326203

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@article{15e64864548b4cb79fc3aef55b41ed9a,

title = "The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation",

abstract = "Purpose: To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. Methods: The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-γ stimulation. Results: RPEs expressed IDO and class II MHC molecules under IFN-γ stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-γ prestimulation. This phenomenon was enhanced by IFN-γ stimulation. The addition of 1-methyl tryptophan (1-MT) into the culture medium successfully reversed RPE immunosuppression. Conclusions: The expression of IDO may in part explain RPE-mediated immune suppression effects.",

keywords = "3-dioxygenase, Co-stimulatory molecule, Immune modulation, Indoleamine 2, Major histocompatibility complex, Retinal pigment epithelium",

author = "Park, {Choul Yong} and Yang, {Seung Ha} and Chuck, {Roy S.} and Gehlbach, {Peter L.} and Park, {Chung Gyu}",

note = "Funding Information: This research was supported by the grant No. R01-2006-000-11158-0 from the Basic Research Program of the Korean Science & Engineering Foundation and by a grant from the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea (Project No. A040004 ). This work was supported by the Korea Research Foundation Grant (MOEHRD, Basic Research Promotion Fund) (KRF-2008-331-E00210). This work was also supported by the Korea Research Foundation Grant (MOEHRD) (KRF-2008-A0173-00008).",

year = "2010",

month = jan,

doi = "10.3109/09273940903326203",

language = "English (US)",

volume = "18",

pages = "24--31",

journal = "Ocular Immunology and Inflammation",

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T1 - The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation

AU - Park, Choul Yong

AU - Yang, Seung Ha

AU - Chuck, Roy S.

AU - Gehlbach, Peter L.

AU - Park, Chung Gyu

N1 - Funding Information: This research was supported by the grant No. R01-2006-000-11158-0 from the Basic Research Program of the Korean Science & Engineering Foundation and by a grant from the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea (Project No. A040004 ). This work was supported by the Korea Research Foundation Grant (MOEHRD, Basic Research Promotion Fund) (KRF-2008-331-E00210). This work was also supported by the Korea Research Foundation Grant (MOEHRD) (KRF-2008-A0173-00008).

PY - 2010/1

Y1 - 2010/1

N2 - Purpose: To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. Methods: The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-γ stimulation. Results: RPEs expressed IDO and class II MHC molecules under IFN-γ stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-γ prestimulation. This phenomenon was enhanced by IFN-γ stimulation. The addition of 1-methyl tryptophan (1-MT) into the culture medium successfully reversed RPE immunosuppression. Conclusions: The expression of IDO may in part explain RPE-mediated immune suppression effects.

AB - Purpose: To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. Methods: The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-γ stimulation. Results: RPEs expressed IDO and class II MHC molecules under IFN-γ stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-γ prestimulation. This phenomenon was enhanced by IFN-γ stimulation. The addition of 1-methyl tryptophan (1-MT) into the culture medium successfully reversed RPE immunosuppression. Conclusions: The expression of IDO may in part explain RPE-mediated immune suppression effects.

KW - 3-dioxygenase

KW - Co-stimulatory molecule

KW - Immune modulation

KW - Indoleamine 2

KW - Major histocompatibility complex

KW - Retinal pigment epithelium

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The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation

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