The role of indoleamine 2,3-dioxygenase in retinal pigment epithelial cell-mediated immune modulation

Choul Yong Park, Seung Ha Yang, Roy S. Chuck, Peter L. Gehlbach, Chung Gyu Park

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Purpose: To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. Methods: The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-γ stimulation. Results: RPEs expressed IDO and class II MHC molecules under IFN-γ stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-γ prestimulation. This phenomenon was enhanced by IFN-γ stimulation. The addition of 1-methyl tryptophan (1-MT) into the culture medium successfully reversed RPE immunosuppression. Conclusions: The expression of IDO may in part explain RPE-mediated immune suppression effects.

Original languageEnglish (US)
Pages (from-to)24-31
Number of pages8
JournalOcular Immunology and Inflammation
Volume18
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

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Keywords

  • 3-dioxygenase
  • Co-stimulatory molecule
  • Immune modulation
  • Indoleamine 2
  • Major histocompatibility complex
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Immunology and Allergy
  • Ophthalmology

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