The role of Ca2+ channels in hippocampal mossy fiber synaptic transmission and long-term potentiation

Pablo E. Castillo, Marc G. Weisskopf, Roger A. Nicoll

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

We have addressed the role of Ca2+ channels in mossy fiber synaptic transmission and long-term potentiation (LTP). Whereas the induction of mossy fiber LTP is entirely normal when synaptic transmission is blocked by the glutamate receptor antagonist kynurenate, LTP is blocked in the absence of extracellular Ca2+. These findings suggest that presynaptic Cal+ entry is essential for mossy fiber LTP. Therefore, the role of different types of presynaptic Ca2+ channels in synaptic transmission and LTP was investigated. Mossy fiber responses were little affected by the L-type Ca2+ channel blocker nifedipine. They were blocked partially by cu-conotoxin-GVIA (N-type) and almost entirely by w-agatoxin-IVA (P-type). None of these antagonists blocked mossy fiber LTP, nor was its expression associated with a change in sensitivity of synaptic transmission to either of the two toxins. These results, together with previous findings, suggest that the induction of mossy fiber LTP is critically dependent on the entry of Ca2+ into the presynaptic terminal to trigger a series of steps resulting in the long lasting enhancement of evoked glutamate release. Whereas P-type Ca2+ channels are of primary importance in mossy fiber synaptic transmission, both the induction and expression of mossy fiber LTP can occur in the absence of P-type (or N-type) Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)261-269
Number of pages9
JournalNeuron
Volume12
Issue number2
DOIs
StatePublished - 1994
Externally publishedYes

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Hippocampal Mossy Fibers
Long-Term Potentiation
Synaptic Transmission
Agatoxins
Conotoxins
Kynurenic Acid
Excitatory Amino Acid Antagonists
Presynaptic Terminals
Nifedipine
Glutamic Acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The role of Ca2+ channels in hippocampal mossy fiber synaptic transmission and long-term potentiation. / Castillo, Pablo E.; Weisskopf, Marc G.; Nicoll, Roger A.

In: Neuron, Vol. 12, No. 2, 1994, p. 261-269.

Research output: Contribution to journalArticle

Castillo, Pablo E. ; Weisskopf, Marc G. ; Nicoll, Roger A. / The role of Ca2+ channels in hippocampal mossy fiber synaptic transmission and long-term potentiation. In: Neuron. 1994 ; Vol. 12, No. 2. pp. 261-269.
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