The role of aquaporin-4 in the blood-brain barrier development and integrity: Studies in animal and cell culture models

G. P. Nicchia, B. Nico, L. M.A. Camassa, M. G. Mola, N. Loh, R. Dermietzel, D. C. Spray, M. Svelto, A. Frigeri

Research output: Contribution to journalArticlepeer-review

180 Scopus citations

Abstract

Aquaporin-4 (AQP4) is the major water channel expressed in brain perivascular astrocyte processes. Although the role of AQP4 in brain edema has been extensively investigated, little information exists regarding its functional role at the blood-brain barrier (BBB). The purpose of this work is to integrate previous and recent data regarding AQP4 expression during BBB formation and depending on BBB integrity, using several experimental models. Results from studies on the chick optic tectum, a well-established model of BBB development, and the effect of lipopolysaccharide on the BBB integrity and on perivascular AQP4 expression have been analyzed and discussed. Moreover, data on the BBB structure and AQP4 expression in murine models of Duchenne muscular dystrophy are reviewed. In particular, published results obtained from mdx 3cv mice have been analyzed together with new data obtained from mdx mice in which all the dystrophin isoforms including DP71 are strongly reduced. Finally, the role of the endothelial component on AQP4 cellular expression and distribution has been investigated using rat primary astrocytes and brain capillary endothelial cell co-cultures as an in vitro model of BBB.

Original languageEnglish (US)
Pages (from-to)935-944
Number of pages10
JournalNeuroscience
Volume129
Issue number4
DOIs
StatePublished - 2004

Keywords

  • aquaporins
  • astrocytes
  • brain edema
  • co-cultures
  • mdx
  • muscular dystrophy

ASJC Scopus subject areas

  • General Neuroscience

Fingerprint

Dive into the research topics of 'The role of aquaporin-4 in the blood-brain barrier development and integrity: Studies in animal and cell culture models'. Together they form a unique fingerprint.

Cite this