The role of albumin in the hepatic transport of bilirubin

Studies in mutant analbuminemic rats

Masayasu Inoue, Etsuko Hirata, Yoshimasa Morino, Sumi Nagase, Jayanta Roy-Chowdhury, Namita R. Chowdhury, Irwin M. Arias

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Bilirubin and other cholephilic organic anions are bound to albumin in the circulation; their hepatic uptake involves a carrier-mediated process. To investigate the possible role of serum albumin in the transhepatic transport of a cholephilic ligand, plasma clearance of radioactive bilirubin and its biliary excretion as well as its interaction with plasma proteins were compared between normal and mutant analbu-minemic rats (NAR). With a tracer amount of 3H-labeled bilirubin, its plasma clearance and biliary excretion were comparable in both animal groups. However, the plasma clearance of a loading dose of the ligand was significantly increased and its biliary recovery was low in NAR as compared with normal animals. In accord with these findings in vivo, gel permeation chromatographic analysis revealed that the bilirubin binding capacity of serum proteins was significantly lower in NAR than in control animals. When bilirubin was administered to NAR as a mixture with equimolar albumin, its plasma disappearance was considerably decreased and its biliary recovery was increased. Similar effects were observed when albumin was replaced by an equimolar amount of glutathione S-transferases (ligandins). These observations indicate that, although ligand-protein interaction in the circulation is important for directing bilirubin to the plasma membranes of the hepatocyte, this mechanism is not specific for albumin.

Original languageEnglish (US)
Pages (from-to)737-743
Number of pages7
JournalJournal of Biochemistry
Volume97
Issue number3
StatePublished - Mar 1985

Fingerprint

Bilirubin
Mutant
Rats
Albumins
Plasma
Plasmas
Clearance
Liver
Animals
Ligands
Proteins
Protein
Glutathione Transferase
Serum Albumin
Recovery
Blood Proteins
Glutathione
Plasma Membrane
Gel permeation chromatography
Beam plasma interactions

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Inoue, M., Hirata, E., Morino, Y., Nagase, S., Roy-Chowdhury, J., Chowdhury, N. R., & Arias, I. M. (1985). The role of albumin in the hepatic transport of bilirubin: Studies in mutant analbuminemic rats. Journal of Biochemistry, 97(3), 737-743.

The role of albumin in the hepatic transport of bilirubin : Studies in mutant analbuminemic rats. / Inoue, Masayasu; Hirata, Etsuko; Morino, Yoshimasa; Nagase, Sumi; Roy-Chowdhury, Jayanta; Chowdhury, Namita R.; Arias, Irwin M.

In: Journal of Biochemistry, Vol. 97, No. 3, 03.1985, p. 737-743.

Research output: Contribution to journalArticle

Inoue, M, Hirata, E, Morino, Y, Nagase, S, Roy-Chowdhury, J, Chowdhury, NR & Arias, IM 1985, 'The role of albumin in the hepatic transport of bilirubin: Studies in mutant analbuminemic rats', Journal of Biochemistry, vol. 97, no. 3, pp. 737-743.
Inoue, Masayasu ; Hirata, Etsuko ; Morino, Yoshimasa ; Nagase, Sumi ; Roy-Chowdhury, Jayanta ; Chowdhury, Namita R. ; Arias, Irwin M. / The role of albumin in the hepatic transport of bilirubin : Studies in mutant analbuminemic rats. In: Journal of Biochemistry. 1985 ; Vol. 97, No. 3. pp. 737-743.
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