The Ro ribonucleoprotein particle: Induction of autoantibodies and the detection of Ro RNAs among species

Mark J. Mamula; Charles A. O'Brien; John B. Harley; John A. Hardin

doi:10.1016/0090-1229(89)90158-X

The Ro ribonucleoprotein particle: Induction of autoantibodies and the detection of Ro RNAs among species

Mark J. Mamula, Charles A. O'Brien, John B. Harley, John A. Hardin

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

High titers of autoantibody specific for the Ro(SSA) ribonucleoprotein are frequently found in patients with systemic lupus erythematosus and Sjogren's syndrome. In this study we have analyzed the immune responses to the Ro particle when utilized as an immunogen in animal hosts. Anti-Ro autoantibodies which bound autologous Ro ribonucleoprotein particles were induced in rabbits. In immunodiffusion studies using crude rabbit tissue extracts, the rabbit antibody made a precipitin line of identity with a prototype human anti-Ro serum. In solid-phase assays, the human autoimmune serum and the antigen-induced rabbit serum competed for similar or overlapping epitopes on the Ro particle. The rabbit and human sera precipitated the four Ro RNAs from human cells as well as four previously uncharacterized Ro RNAs from a bovine cell line, three Ro RNAs from a rabbit cell line, and two Ro RNAs from duck cells. While total numbers of cellular Ro RNAs differ among species, all possess an RNA of common size which comigrated with the hY1 of human cells.

Original language	English (US)
Pages (from-to)	435-446
Number of pages	12
Journal	Clinical Immunology and Immunopathology
Volume	52
Issue number	3
DOIs	https://doi.org/10.1016/0090-1229(89)90158-X
State	Published - Sep 1989
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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title = "The Ro ribonucleoprotein particle: Induction of autoantibodies and the detection of Ro RNAs among species",

abstract = "High titers of autoantibody specific for the Ro(SSA) ribonucleoprotein are frequently found in patients with systemic lupus erythematosus and Sjogren's syndrome. In this study we have analyzed the immune responses to the Ro particle when utilized as an immunogen in animal hosts. Anti-Ro autoantibodies which bound autologous Ro ribonucleoprotein particles were induced in rabbits. In immunodiffusion studies using crude rabbit tissue extracts, the rabbit antibody made a precipitin line of identity with a prototype human anti-Ro serum. In solid-phase assays, the human autoimmune serum and the antigen-induced rabbit serum competed for similar or overlapping epitopes on the Ro particle. The rabbit and human sera precipitated the four Ro RNAs from human cells as well as four previously uncharacterized Ro RNAs from a bovine cell line, three Ro RNAs from a rabbit cell line, and two Ro RNAs from duck cells. While total numbers of cellular Ro RNAs differ among species, all possess an RNA of common size which comigrated with the hY1 of human cells.",

author = "Mamula, {Mark J.} and O'Brien, {Charles A.} and Harley, {John B.} and Hardin, {John A.}",

note = "Funding Information: This study was supported by the National Institutes of Health Grants AM-3149 and AI-24717, the Veteran{\textquoteright}s Administration, and an Arthritis Center grant from the Arthritis Foundation. Dr. Mamma is a recipient of a Gina Finzi Memorial Fellowship from the Lupus Foundation of America and a Postdoctoral Fellowship from the Arthritis Foundation. We thank Dr. Joseph Craft for his contributions of technical expertise and critical appraisal of this manuscript.",

year = "1989",

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doi = "10.1016/0090-1229(89)90158-X",

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AU - O'Brien, Charles A.

AU - Harley, John B.

AU - Hardin, John A.

N1 - Funding Information: This study was supported by the National Institutes of Health Grants AM-3149 and AI-24717, the Veteran’s Administration, and an Arthritis Center grant from the Arthritis Foundation. Dr. Mamma is a recipient of a Gina Finzi Memorial Fellowship from the Lupus Foundation of America and a Postdoctoral Fellowship from the Arthritis Foundation. We thank Dr. Joseph Craft for his contributions of technical expertise and critical appraisal of this manuscript.

PY - 1989/9

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N2 - High titers of autoantibody specific for the Ro(SSA) ribonucleoprotein are frequently found in patients with systemic lupus erythematosus and Sjogren's syndrome. In this study we have analyzed the immune responses to the Ro particle when utilized as an immunogen in animal hosts. Anti-Ro autoantibodies which bound autologous Ro ribonucleoprotein particles were induced in rabbits. In immunodiffusion studies using crude rabbit tissue extracts, the rabbit antibody made a precipitin line of identity with a prototype human anti-Ro serum. In solid-phase assays, the human autoimmune serum and the antigen-induced rabbit serum competed for similar or overlapping epitopes on the Ro particle. The rabbit and human sera precipitated the four Ro RNAs from human cells as well as four previously uncharacterized Ro RNAs from a bovine cell line, three Ro RNAs from a rabbit cell line, and two Ro RNAs from duck cells. While total numbers of cellular Ro RNAs differ among species, all possess an RNA of common size which comigrated with the hY1 of human cells.

AB - High titers of autoantibody specific for the Ro(SSA) ribonucleoprotein are frequently found in patients with systemic lupus erythematosus and Sjogren's syndrome. In this study we have analyzed the immune responses to the Ro particle when utilized as an immunogen in animal hosts. Anti-Ro autoantibodies which bound autologous Ro ribonucleoprotein particles were induced in rabbits. In immunodiffusion studies using crude rabbit tissue extracts, the rabbit antibody made a precipitin line of identity with a prototype human anti-Ro serum. In solid-phase assays, the human autoimmune serum and the antigen-induced rabbit serum competed for similar or overlapping epitopes on the Ro particle. The rabbit and human sera precipitated the four Ro RNAs from human cells as well as four previously uncharacterized Ro RNAs from a bovine cell line, three Ro RNAs from a rabbit cell line, and two Ro RNAs from duck cells. While total numbers of cellular Ro RNAs differ among species, all possess an RNA of common size which comigrated with the hY1 of human cells.

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The Ro ribonucleoprotein particle: Induction of autoantibodies and the detection of Ro RNAs among species

Abstract

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