The removal of UV-induced pyrimidine dimers from DNA of rat skin cells in vitro and in vivo in relation to aging

E. Mullaart, L. Roza, P. H M Lohman, Jan Vijg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Young and old rats were compared with respect to the capacity of their skin fibroblasts and epidermal keratinocytes to remove low levels of ultraviolet light (UV) induced UV-endonuclease sensitive sites (pyrimidine dimers) from their DNA, in vitro and in vivo, respectively. In vitro, over a 24-h time period, fibroblasts from both young and old rats were found to remove about 20% of the pyrimidine dimers originally induced by 4.6 J/m2 of UV-C. In vivo, after 2.6 kJ/m2 of UV-B hardly any UV lesions were found to be present in fibroblasts, as demonstrated by immunohistochemistry using an anti-thymine dimer antibody. As reported earlier (Mullaart et a., J. Invest. Dermatol., 90 (1988) 346-349) cultured epidermal keratinocytes do not differ from cultured fibroblasts in UV repair kinetics, whereas in vivo they remove at least 50% of the pyrimidine dimers induced by 4 kJ/m2 of UV-B within 3 h. We now show that epidermal keratinocytes from old rats are not deficient in their in vivo repair characteristics upon this low UV-B dose. However, since a considerable fraction of the pyrimidine dimers appeared to be persistent in fibroblasts and keratinocytes, demonstrated by both enzymatic and immunochemical assays, the possibility is discussed that long-term exposure of skin cells to UV may lead to an accumulation of DNA damage with age.

Original languageEnglish (US)
Pages (from-to)253-264
Number of pages12
JournalMechanisms of Ageing and Development
Volume47
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Pyrimidine Dimers
Ultraviolet Rays
Rats
Skin
Aging of materials
Fibroblasts
DNA
Keratinocytes
Repair
In Vitro Techniques
Endonucleases
Enzyme Assays
DNA Damage
Assays
Immunohistochemistry
Kinetics

Keywords

  • Aging
  • DNA repair
  • Persisting DNA damage
  • Rat skin cells
  • UV-induced pyrimidine dimers

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

The removal of UV-induced pyrimidine dimers from DNA of rat skin cells in vitro and in vivo in relation to aging. / Mullaart, E.; Roza, L.; Lohman, P. H M; Vijg, Jan.

In: Mechanisms of Ageing and Development, Vol. 47, No. 3, 1989, p. 253-264.

Research output: Contribution to journalArticle

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