The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism

Patrick J. Keeling, Nicolas Corradi, Hilary G. Morrison, Karen L. Haag, Dieter Ebert, Louis M. Weiss, Donna E. Akiyoshi, Saul Tzipori

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Reduction of various biological processes is a hallmark of the parasitic lifestyle. Generally, the more intimate the association between parasites and hosts the stronger the parasite relies on its host's physiology for survival and reproduction. However, some systems have been held to be indispensable, for example, the core pathways of carbon metabolism that produce energy from sugars. Even the most hardened anaerobes that lack oxidative phosphorylation and the tricarboxylic acid cycle have retained glycolysis and some downstream means to generate ATP. Here we describe the deep-coverage genome resequencing of the pathogenic microsporidiian, Enterocytozoon bieneusi, which shows that this parasite has crossed this line and abandoned complete pathways for the most basic carbon metabolism. Comparing two genome sequence surveys of E. bieneusi to genomic data from four other microsporidia reveals a normal complement of 353 genes representing 30 functional pathways in E. bieneusi, except that only 2 out of 21 genes collectively involved in glycolysis, pentose phosphate, and trehalose metabolism are present. Similarly, no genes encoding proteins involved in the processing of spliceosomal introns were found. Altogether, E. bieneusi appears to have no fully functional pathway to generate ATP from glucose. Therefore, this intracellular parasite relies on transporters to import ATP from its host.

Original languageEnglish (US)
Pages (from-to)304-309
Number of pages6
JournalGenome Biology and Evolution
Volume2
Issue number1
DOIs
StatePublished - 2010

Fingerprint

Enterocytozoon
Microsporidia
parasite
Parasites
Carbon
genome
metabolism
Genome
parasites
gene
carbon
Adenosine Triphosphate
glycolysis
Glycolysis
Genes
genes
Pentoses
Biological Phenomena
Trehalose
anaerobes

Keywords

  • Carbon metabolism
  • Evolution
  • Glycolysis
  • Microsporidia
  • Parasite
  • Reduction

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism. / Keeling, Patrick J.; Corradi, Nicolas; Morrison, Hilary G.; Haag, Karen L.; Ebert, Dieter; Weiss, Louis M.; Akiyoshi, Donna E.; Tzipori, Saul.

In: Genome Biology and Evolution, Vol. 2, No. 1, 2010, p. 304-309.

Research output: Contribution to journalArticle

Keeling, PJ, Corradi, N, Morrison, HG, Haag, KL, Ebert, D, Weiss, LM, Akiyoshi, DE & Tzipori, S 2010, 'The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism', Genome Biology and Evolution, vol. 2, no. 1, pp. 304-309. https://doi.org/10.1093/gbe/evq022
Keeling, Patrick J. ; Corradi, Nicolas ; Morrison, Hilary G. ; Haag, Karen L. ; Ebert, Dieter ; Weiss, Louis M. ; Akiyoshi, Donna E. ; Tzipori, Saul. / The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism. In: Genome Biology and Evolution. 2010 ; Vol. 2, No. 1. pp. 304-309.
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