Abstract
Cholesterol is a major component of biological membranes, yet there is very little information concerning its distribution across the membrane. Recent experiments in our laboratory, using cholesterol oxidase, have demonstrated that cholesterol can undergo a rapid transbilayer movement in lecithin-cholesterol vesicles in a half-time of 1 min or less at 37°C. In order to support this conclusion, we have sought other approaches to the measurement of this process. We now report our finding that the transbilayer movement of thiocholesterol in phospholipid vesicles occurs in a half-time of 1 min or less at 20°C.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 373-375 |
| Number of pages | 3 |
| Journal | BBA - Biomembranes |
| Volume | 644 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 22 1981 |
| Externally published | Yes |
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Keywords
- Phospholipid vesicle
- Thiocholesterol
- Transbilayer movement
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Medicine(all)
Cite this
The rapid transbilayer movement of thiocholesterol in small unilamellar phospholipid vesicles. / Dawidowicz, Eliezar A.; Backer, Jonathan M.
In: BBA - Biomembranes, Vol. 644, No. 2, 22.06.1981, p. 373-375.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The rapid transbilayer movement of thiocholesterol in small unilamellar phospholipid vesicles
AU - Dawidowicz, Eliezar A.
AU - Backer, Jonathan M.
PY - 1981/6/22
Y1 - 1981/6/22
N2 - Cholesterol is a major component of biological membranes, yet there is very little information concerning its distribution across the membrane. Recent experiments in our laboratory, using cholesterol oxidase, have demonstrated that cholesterol can undergo a rapid transbilayer movement in lecithin-cholesterol vesicles in a half-time of 1 min or less at 37°C. In order to support this conclusion, we have sought other approaches to the measurement of this process. We now report our finding that the transbilayer movement of thiocholesterol in phospholipid vesicles occurs in a half-time of 1 min or less at 20°C.
AB - Cholesterol is a major component of biological membranes, yet there is very little information concerning its distribution across the membrane. Recent experiments in our laboratory, using cholesterol oxidase, have demonstrated that cholesterol can undergo a rapid transbilayer movement in lecithin-cholesterol vesicles in a half-time of 1 min or less at 37°C. In order to support this conclusion, we have sought other approaches to the measurement of this process. We now report our finding that the transbilayer movement of thiocholesterol in phospholipid vesicles occurs in a half-time of 1 min or less at 20°C.
KW - Phospholipid vesicle
KW - Thiocholesterol
KW - Transbilayer movement
UR - http://www.scopus.com/inward/record.url?scp=0019886348&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019886348&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(81)90396-5
DO - 10.1016/0005-2736(81)90396-5
M3 - Article
C2 - 7260079
AN - SCOPUS:0019886348
VL - 644
SP - 373
EP - 375
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -