TY - JOUR
T1 - The rapid transbilayer movement of thiocholesterol in small unilamellar phospholipid vesicles
AU - Dawidowicz, Eliezar A.
AU - Backer, Jonathan M.
N1 - Funding Information:
We wish to thank Debra Williams for here excellent assistance. This work was supported by a grant
Funding Information:
from the U.S. Public Health Service (GM 22175) and by a grant-in-aid from the American Heart Association (77-1066) and with funds contributed in part by the Massachusetts Heart Association.
PY - 1981/6/22
Y1 - 1981/6/22
N2 - Cholesterol is a major component of biological membranes, yet there is very little information concerning its distribution across the membrane. Recent experiments in our laboratory, using cholesterol oxidase, have demonstrated that cholesterol can undergo a rapid transbilayer movement in lecithin-cholesterol vesicles in a half-time of 1 min or less at 37°C. In order to support this conclusion, we have sought other approaches to the measurement of this process. We now report our finding that the transbilayer movement of thiocholesterol in phospholipid vesicles occurs in a half-time of 1 min or less at 20°C.
AB - Cholesterol is a major component of biological membranes, yet there is very little information concerning its distribution across the membrane. Recent experiments in our laboratory, using cholesterol oxidase, have demonstrated that cholesterol can undergo a rapid transbilayer movement in lecithin-cholesterol vesicles in a half-time of 1 min or less at 37°C. In order to support this conclusion, we have sought other approaches to the measurement of this process. We now report our finding that the transbilayer movement of thiocholesterol in phospholipid vesicles occurs in a half-time of 1 min or less at 20°C.
KW - Phospholipid vesicle
KW - Thiocholesterol
KW - Transbilayer movement
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U2 - 10.1016/0005-2736(81)90396-5
DO - 10.1016/0005-2736(81)90396-5
M3 - Article
C2 - 7260079
AN - SCOPUS:0019886348
VL - 644
SP - 373
EP - 375
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -