TY - JOUR
T1 - The quorum sensing molecule N-acyl homoserine lactone produced by Acinetobacter baumannii displays antibacterial and anticancer properties
AU - John, James
AU - Saranathan, Rajagopalan
AU - Adigopula, Lakshmi Narayana
AU - Thamodharan, Vasanth
AU - Singh, Satya Prakash
AU - Pragna Lakshmi, T.
AU - CharanTej, Mallu Abhiram
AU - Srinivasa Rao, R.
AU - Krishna, R.
AU - Surya Prakash Rao, H.
AU - Prashanth, K.
N1 - Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2016/8/18
Y1 - 2016/8/18
N2 - Secretory N-acyl homoserine lactones (AHLs) mediate quorum sensing (QS) in bacteria. AHLs are shown to be inhibitory for an unrelated group of bacteria and might mimic host signalling elements, thereby subverting the regulatory events in host cells. This study investigated the AHL produced by Acinetobacter baumannii and analysed its effect on other bacterial species and mammalian cells. Chemically characterized AHL had an m/z value of 325 with a molecular formula C18H31NO4 and showed its inhibitory potential against Staphylococcus aureus. Molecular docking studies identified D-alanine-D-alanine synthetase A, a cell wall synthesizing enzyme of S. aureus having a strong binding affinity towards AHL. Electron microscopy showed the disruption and sloughing off of the S. aureus cell wall when treated with AHL. In vitro experiments revealed that this bacteriostatic AHL showed time-dependent activity and induced apoptosis in cancer cell lines. This compound could be a potential structural backbone for constructing new AHL analogues against S. aureus. The findings emphasize the need to re-evaluate all previously characterized AHLs for any additional new biological functions other than QS.
AB - Secretory N-acyl homoserine lactones (AHLs) mediate quorum sensing (QS) in bacteria. AHLs are shown to be inhibitory for an unrelated group of bacteria and might mimic host signalling elements, thereby subverting the regulatory events in host cells. This study investigated the AHL produced by Acinetobacter baumannii and analysed its effect on other bacterial species and mammalian cells. Chemically characterized AHL had an m/z value of 325 with a molecular formula C18H31NO4 and showed its inhibitory potential against Staphylococcus aureus. Molecular docking studies identified D-alanine-D-alanine synthetase A, a cell wall synthesizing enzyme of S. aureus having a strong binding affinity towards AHL. Electron microscopy showed the disruption and sloughing off of the S. aureus cell wall when treated with AHL. In vitro experiments revealed that this bacteriostatic AHL showed time-dependent activity and induced apoptosis in cancer cell lines. This compound could be a potential structural backbone for constructing new AHL analogues against S. aureus. The findings emphasize the need to re-evaluate all previously characterized AHLs for any additional new biological functions other than QS.
KW - Acinetobacter baumannii
KW - D-alanine-D-alanine synthetase A
KW - N-acyl homoserine lactone
KW - Quorum sensing
KW - Staphylococcus aureus
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U2 - 10.1080/08927014.2016.1221946
DO - 10.1080/08927014.2016.1221946
M3 - Article
C2 - 27643959
AN - SCOPUS:84988874534
SN - 0892-7014
VL - 32
SP - 1029
EP - 1047
JO - Biofouling
JF - Biofouling
IS - 9
ER -