The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans

Debora L. Oliveira; Fernanda L. Fonseca; Daniel Zamith-Miranda; Leonardo Nimrichter; Jéssica Rodrigues; Marcos D. Pereira; Julia C.V. Reuwsaat; Augusto Schrank; Charley Staats; Livia Kmetzsch; Marilene H. Vainstein; Marcio L. Rodrigues

doi:10.2217/fmb-2016-0090

The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans

Debora L. Oliveira, Fernanda L. Fonseca, Daniel Zamith-Miranda, Leonardo Nimrichter, Jéssica Rodrigues, Marcos D. Pereira, Julia C.V. Reuwsaat, Augusto Schrank, Charley Staats, Livia Kmetzsch, Marilene H. Vainstein, Marcio L. Rodrigues

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Aim: We investigated the involvement of the autophagy protein 7 (Atg7) in physiology and pathogenic potential of Cryptococcus neoformans. Materials & methods: The C. neoformans gene encoding Atg7 was deleted by biolistic transformation for characterization of autophagy mechanisms, pigment formation, cell dimensions, interaction with phagocytes and pathogenic potential in vivo. Results & conclusion: ATG7 deletion resulted in defective autophagy mechanisms, enhanced pigmentation and increased cellular size both in vitro and in vivo. The atg7Δ mutant had decreased survival in the lung of infected mice, higher susceptibility to the killing machinery of different host phagocytes and reduced ability to kill an invertebrate host. These results connect Atg7 with mechanisms of pathogenicity in the C. neoformans model.

Original language	English (US)
Pages (from-to)	1405-1419
Number of pages	15
Journal	Future Microbiology
Volume	11
Issue number	11
DOIs	https://doi.org/10.2217/fmb-2016-0090
State	Published - Nov 2016
Externally published	Yes

Keywords

ATG7
autophagy
Cryptococcus neoformans
macrophages
virulence

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

Access to Document

10.2217/fmb-2016-0090

Cite this

Oliveira, D. L., Fonseca, F. L., Zamith-Miranda, D., Nimrichter, L., Rodrigues, J., Pereira, M. D., Reuwsaat, J. C. V., Schrank, A., Staats, C., Kmetzsch, L., Vainstein, M. H., & Rodrigues, M. L. (2016). The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans. Future Microbiology, 11(11), 1405-1419. https://doi.org/10.2217/fmb-2016-0090

Oliveira, DL, Fonseca, FL, Zamith-Miranda, D, Nimrichter, L, Rodrigues, J, Pereira, MD, Reuwsaat, JCV, Schrank, A, Staats, C, Kmetzsch, L, Vainstein, MH & Rodrigues, ML 2016, 'The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans', Future Microbiology, vol. 11, no. 11, pp. 1405-1419. https://doi.org/10.2217/fmb-2016-0090

@article{354e77478769446998144f40793cea29,

title = "The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans",

abstract = "Aim: We investigated the involvement of the autophagy protein 7 (Atg7) in physiology and pathogenic potential of Cryptococcus neoformans. Materials & methods: The C. neoformans gene encoding Atg7 was deleted by biolistic transformation for characterization of autophagy mechanisms, pigment formation, cell dimensions, interaction with phagocytes and pathogenic potential in vivo. Results & conclusion: ATG7 deletion resulted in defective autophagy mechanisms, enhanced pigmentation and increased cellular size both in vitro and in vivo. The atg7Δ mutant had decreased survival in the lung of infected mice, higher susceptibility to the killing machinery of different host phagocytes and reduced ability to kill an invertebrate host. These results connect Atg7 with mechanisms of pathogenicity in the C. neoformans model.",

keywords = "ATG7, autophagy, Cryptococcus neoformans, macrophages, virulence",

author = "Oliveira, {Debora L.} and Fonseca, {Fernanda L.} and Daniel Zamith-Miranda and Leonardo Nimrichter and J{\'e}ssica Rodrigues and Pereira, {Marcos D.} and Reuwsaat, {Julia C.V.} and Augusto Schrank and Charley Staats and Livia Kmetzsch and Vainstein, {Marilene H.} and Rodrigues, {Marcio L.}",

year = "2016",

month = nov,

doi = "10.2217/fmb-2016-0090",

language = "English (US)",

volume = "11",

pages = "1405--1419",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Future Medicine Ltd.",

number = "11",

}

TY - JOUR

T1 - The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans

AU - Oliveira, Debora L.

AU - Fonseca, Fernanda L.

AU - Zamith-Miranda, Daniel

AU - Nimrichter, Leonardo

AU - Rodrigues, Jéssica

AU - Pereira, Marcos D.

AU - Reuwsaat, Julia C.V.

AU - Schrank, Augusto

AU - Staats, Charley

AU - Kmetzsch, Livia

AU - Vainstein, Marilene H.

AU - Rodrigues, Marcio L.

PY - 2016/11

Y1 - 2016/11

N2 - Aim: We investigated the involvement of the autophagy protein 7 (Atg7) in physiology and pathogenic potential of Cryptococcus neoformans. Materials & methods: The C. neoformans gene encoding Atg7 was deleted by biolistic transformation for characterization of autophagy mechanisms, pigment formation, cell dimensions, interaction with phagocytes and pathogenic potential in vivo. Results & conclusion: ATG7 deletion resulted in defective autophagy mechanisms, enhanced pigmentation and increased cellular size both in vitro and in vivo. The atg7Δ mutant had decreased survival in the lung of infected mice, higher susceptibility to the killing machinery of different host phagocytes and reduced ability to kill an invertebrate host. These results connect Atg7 with mechanisms of pathogenicity in the C. neoformans model.

AB - Aim: We investigated the involvement of the autophagy protein 7 (Atg7) in physiology and pathogenic potential of Cryptococcus neoformans. Materials & methods: The C. neoformans gene encoding Atg7 was deleted by biolistic transformation for characterization of autophagy mechanisms, pigment formation, cell dimensions, interaction with phagocytes and pathogenic potential in vivo. Results & conclusion: ATG7 deletion resulted in defective autophagy mechanisms, enhanced pigmentation and increased cellular size both in vitro and in vivo. The atg7Δ mutant had decreased survival in the lung of infected mice, higher susceptibility to the killing machinery of different host phagocytes and reduced ability to kill an invertebrate host. These results connect Atg7 with mechanisms of pathogenicity in the C. neoformans model.

KW - ATG7

KW - autophagy

KW - Cryptococcus neoformans

KW - macrophages

KW - virulence

UR - http://www.scopus.com/inward/record.url?scp=84994156600&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994156600&partnerID=8YFLogxK

U2 - 10.2217/fmb-2016-0090

DO - 10.2217/fmb-2016-0090

M3 - Article

C2 - 27750454

AN - SCOPUS:84994156600

SN - 1746-0913

VL - 11

SP - 1405

EP - 1419

JO - Future Microbiology

JF - Future Microbiology

IS - 11

ER -

The putative autophagy regulator Atg7 affects the physiology and pathogenic mechanisms of Cryptococcus neoformans

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this