The prostatic utricle is not a müllerian duct remnant: Immunohistochemical evidence for a distinct urogenital sinus origin

Ellen Shapiro, Hongying Huang, Deborah E. McFadden, Rachel J. Masch, Eliza Ng, Herbert Lepor, Xue Ru Wu, Antoine Khoury

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose: The embryological origin of the utricle is thought to be a remnant of the fused caudal ends of the müllerian ducts (MDs). Others propose that the urogenital sinus (UGS) contributes either partially or totally to the development of this structure. Using immunohistochemical probes, we provide strong evidence that the utricle is of UGS origin only. Materials and Methods: Human fetal prostates, gestational ages 9 to 24 weeks, were serially cross-sectioned. Representative sections were stained with antibodies to p63 (basal cell marker), vimentin (mesoderm marker), uroplakins (marker for urothelium) Pax-2 (expressed in ductal and mesenchyme of urogenital system including the MDs and wolffian ducts) and Ki67 (proliferation). Apoptosis was detected with the TUNEL assay. Results: By 9 weeks there was weak expression of p63 in the basal layer of the UGS. At 11 weeks there was increased staining of p63 in the UGS and some p63 staining of the fused MDs, which expressed Pax-2 at this time. At 14 to 15 weeks as the MDs were undergoing apoptosis, there was an ingrowth of uroplakin-expressing UGS epithelium into the periurethral stroma, which formed a plate of p63 positive cells just beneath the UGS that was Ki67 positive. The remaining caudal MD epithelium was p63 negative and expressed vimentin and Pax-2. By 17 weeks the plate of p63 positive cells elongated forming the utricle that remained p63 positive but Pax-2 and vimentin negative. Conclusions: We show that the utricle forms as an ingrowth of specialized cells from the dorsal wall of the UGS as the caudal MDs regress.

Original languageEnglish (US)
Pages (from-to)1753-1756
Number of pages4
JournalJournal of Urology
Volume172
Issue number4 II
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Saccule and Utricle
Uroplakins
Vimentin
Mesoderm
Epithelium
Wolffian Ducts
Apoptosis
Staining and Labeling
Urogenital System
Urothelium
In Situ Nick-End Labeling
Cell Wall
Gestational Age
Prostate
Antibodies

Keywords

  • Embryology
  • Mullerian ducts

ASJC Scopus subject areas

  • Urology

Cite this

The prostatic utricle is not a müllerian duct remnant : Immunohistochemical evidence for a distinct urogenital sinus origin. / Shapiro, Ellen; Huang, Hongying; McFadden, Deborah E.; Masch, Rachel J.; Ng, Eliza; Lepor, Herbert; Wu, Xue Ru; Khoury, Antoine.

In: Journal of Urology, Vol. 172, No. 4 II, 10.2004, p. 1753-1756.

Research output: Contribution to journalArticle

Shapiro, E, Huang, H, McFadden, DE, Masch, RJ, Ng, E, Lepor, H, Wu, XR & Khoury, A 2004, 'The prostatic utricle is not a müllerian duct remnant: Immunohistochemical evidence for a distinct urogenital sinus origin', Journal of Urology, vol. 172, no. 4 II, pp. 1753-1756. https://doi.org/10.1097/01.ju.0000140267.46772.7d
Shapiro, Ellen ; Huang, Hongying ; McFadden, Deborah E. ; Masch, Rachel J. ; Ng, Eliza ; Lepor, Herbert ; Wu, Xue Ru ; Khoury, Antoine. / The prostatic utricle is not a müllerian duct remnant : Immunohistochemical evidence for a distinct urogenital sinus origin. In: Journal of Urology. 2004 ; Vol. 172, No. 4 II. pp. 1753-1756.
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abstract = "Purpose: The embryological origin of the utricle is thought to be a remnant of the fused caudal ends of the m{\"u}llerian ducts (MDs). Others propose that the urogenital sinus (UGS) contributes either partially or totally to the development of this structure. Using immunohistochemical probes, we provide strong evidence that the utricle is of UGS origin only. Materials and Methods: Human fetal prostates, gestational ages 9 to 24 weeks, were serially cross-sectioned. Representative sections were stained with antibodies to p63 (basal cell marker), vimentin (mesoderm marker), uroplakins (marker for urothelium) Pax-2 (expressed in ductal and mesenchyme of urogenital system including the MDs and wolffian ducts) and Ki67 (proliferation). Apoptosis was detected with the TUNEL assay. Results: By 9 weeks there was weak expression of p63 in the basal layer of the UGS. At 11 weeks there was increased staining of p63 in the UGS and some p63 staining of the fused MDs, which expressed Pax-2 at this time. At 14 to 15 weeks as the MDs were undergoing apoptosis, there was an ingrowth of uroplakin-expressing UGS epithelium into the periurethral stroma, which formed a plate of p63 positive cells just beneath the UGS that was Ki67 positive. The remaining caudal MD epithelium was p63 negative and expressed vimentin and Pax-2. By 17 weeks the plate of p63 positive cells elongated forming the utricle that remained p63 positive but Pax-2 and vimentin negative. Conclusions: We show that the utricle forms as an ingrowth of specialized cells from the dorsal wall of the UGS as the caudal MDs regress.",
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T1 - The prostatic utricle is not a müllerian duct remnant

T2 - Immunohistochemical evidence for a distinct urogenital sinus origin

AU - Shapiro, Ellen

AU - Huang, Hongying

AU - McFadden, Deborah E.

AU - Masch, Rachel J.

AU - Ng, Eliza

AU - Lepor, Herbert

AU - Wu, Xue Ru

AU - Khoury, Antoine

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N2 - Purpose: The embryological origin of the utricle is thought to be a remnant of the fused caudal ends of the müllerian ducts (MDs). Others propose that the urogenital sinus (UGS) contributes either partially or totally to the development of this structure. Using immunohistochemical probes, we provide strong evidence that the utricle is of UGS origin only. Materials and Methods: Human fetal prostates, gestational ages 9 to 24 weeks, were serially cross-sectioned. Representative sections were stained with antibodies to p63 (basal cell marker), vimentin (mesoderm marker), uroplakins (marker for urothelium) Pax-2 (expressed in ductal and mesenchyme of urogenital system including the MDs and wolffian ducts) and Ki67 (proliferation). Apoptosis was detected with the TUNEL assay. Results: By 9 weeks there was weak expression of p63 in the basal layer of the UGS. At 11 weeks there was increased staining of p63 in the UGS and some p63 staining of the fused MDs, which expressed Pax-2 at this time. At 14 to 15 weeks as the MDs were undergoing apoptosis, there was an ingrowth of uroplakin-expressing UGS epithelium into the periurethral stroma, which formed a plate of p63 positive cells just beneath the UGS that was Ki67 positive. The remaining caudal MD epithelium was p63 negative and expressed vimentin and Pax-2. By 17 weeks the plate of p63 positive cells elongated forming the utricle that remained p63 positive but Pax-2 and vimentin negative. Conclusions: We show that the utricle forms as an ingrowth of specialized cells from the dorsal wall of the UGS as the caudal MDs regress.

AB - Purpose: The embryological origin of the utricle is thought to be a remnant of the fused caudal ends of the müllerian ducts (MDs). Others propose that the urogenital sinus (UGS) contributes either partially or totally to the development of this structure. Using immunohistochemical probes, we provide strong evidence that the utricle is of UGS origin only. Materials and Methods: Human fetal prostates, gestational ages 9 to 24 weeks, were serially cross-sectioned. Representative sections were stained with antibodies to p63 (basal cell marker), vimentin (mesoderm marker), uroplakins (marker for urothelium) Pax-2 (expressed in ductal and mesenchyme of urogenital system including the MDs and wolffian ducts) and Ki67 (proliferation). Apoptosis was detected with the TUNEL assay. Results: By 9 weeks there was weak expression of p63 in the basal layer of the UGS. At 11 weeks there was increased staining of p63 in the UGS and some p63 staining of the fused MDs, which expressed Pax-2 at this time. At 14 to 15 weeks as the MDs were undergoing apoptosis, there was an ingrowth of uroplakin-expressing UGS epithelium into the periurethral stroma, which formed a plate of p63 positive cells just beneath the UGS that was Ki67 positive. The remaining caudal MD epithelium was p63 negative and expressed vimentin and Pax-2. By 17 weeks the plate of p63 positive cells elongated forming the utricle that remained p63 positive but Pax-2 and vimentin negative. Conclusions: We show that the utricle forms as an ingrowth of specialized cells from the dorsal wall of the UGS as the caudal MDs regress.

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