The prostaglandin transporter PGT transports PGH2

Yuling Chi, Victor L. Schuster

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prostaglandin H2 not only serves as the common precursor of all other PGs, but also directly triggers signals (e.g. platelet aggregation), depending on its location and translocation. The prostaglandin carrier PGT mediates the transport of several prostanoids, such as PGE2, and PGF. Here we used PGT in the plasma membrane as a model system to test the hypothesis that PGT also transports PGH2. Using wild-type and PGT-expressing MDCK cells, we show that PGH2 uptake is mediated both by simple diffusion and by PGT. The PGH2 influx permeability coefficient for diffusion is (5.66 ± 0.63) × 10-6 cm/s. The kinetic parameters of PGH2 transport by PGT are Km = 376 ± 34 nM and Vmax = 210.2 ± 11.4 fmol/mg protein/s. PGH2 transport by PGT can be inhibited by excess PGE2 or by a PGT inhibitor. We conclude that PGT may play a role in transporting PGH2 across cellular membranes.

Original languageEnglish (US)
Pages (from-to)168-172
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume395
Issue number2
DOIs
StatePublished - Apr 30 2010

Fingerprint

Prostaglandin H2
Prostaglandins
Dinoprostone
Madin Darby Canine Kidney Cells
Dinoprost
Hydraulic conductivity
Cell membranes
Platelets
Platelet Aggregation
Kinetic parameters
Permeability
Agglomeration
Cell Membrane
Membranes

Keywords

  • Kinetics
  • Prostaglandin H
  • Prostaglandin transporter

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

The prostaglandin transporter PGT transports PGH2. / Chi, Yuling; Schuster, Victor L.

In: Biochemical and Biophysical Research Communications, Vol. 395, No. 2, 30.04.2010, p. 168-172.

Research output: Contribution to journalArticle

@article{b0bf9b0e05d64374ad0c71946c37da35,
title = "The prostaglandin transporter PGT transports PGH2",
abstract = "Prostaglandin H2 not only serves as the common precursor of all other PGs, but also directly triggers signals (e.g. platelet aggregation), depending on its location and translocation. The prostaglandin carrier PGT mediates the transport of several prostanoids, such as PGE2, and PGF2α. Here we used PGT in the plasma membrane as a model system to test the hypothesis that PGT also transports PGH2. Using wild-type and PGT-expressing MDCK cells, we show that PGH2 uptake is mediated both by simple diffusion and by PGT. The PGH2 influx permeability coefficient for diffusion is (5.66 ± 0.63) × 10-6 cm/s. The kinetic parameters of PGH2 transport by PGT are Km = 376 ± 34 nM and Vmax = 210.2 ± 11.4 fmol/mg protein/s. PGH2 transport by PGT can be inhibited by excess PGE2 or by a PGT inhibitor. We conclude that PGT may play a role in transporting PGH2 across cellular membranes.",
keywords = "Kinetics, Prostaglandin H, Prostaglandin transporter",
author = "Yuling Chi and Schuster, {Victor L.}",
year = "2010",
month = "4",
day = "30",
doi = "10.1016/j.bbrc.2010.03.108",
language = "English (US)",
volume = "395",
pages = "168--172",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The prostaglandin transporter PGT transports PGH2

AU - Chi, Yuling

AU - Schuster, Victor L.

PY - 2010/4/30

Y1 - 2010/4/30

N2 - Prostaglandin H2 not only serves as the common precursor of all other PGs, but also directly triggers signals (e.g. platelet aggregation), depending on its location and translocation. The prostaglandin carrier PGT mediates the transport of several prostanoids, such as PGE2, and PGF2α. Here we used PGT in the plasma membrane as a model system to test the hypothesis that PGT also transports PGH2. Using wild-type and PGT-expressing MDCK cells, we show that PGH2 uptake is mediated both by simple diffusion and by PGT. The PGH2 influx permeability coefficient for diffusion is (5.66 ± 0.63) × 10-6 cm/s. The kinetic parameters of PGH2 transport by PGT are Km = 376 ± 34 nM and Vmax = 210.2 ± 11.4 fmol/mg protein/s. PGH2 transport by PGT can be inhibited by excess PGE2 or by a PGT inhibitor. We conclude that PGT may play a role in transporting PGH2 across cellular membranes.

AB - Prostaglandin H2 not only serves as the common precursor of all other PGs, but also directly triggers signals (e.g. platelet aggregation), depending on its location and translocation. The prostaglandin carrier PGT mediates the transport of several prostanoids, such as PGE2, and PGF2α. Here we used PGT in the plasma membrane as a model system to test the hypothesis that PGT also transports PGH2. Using wild-type and PGT-expressing MDCK cells, we show that PGH2 uptake is mediated both by simple diffusion and by PGT. The PGH2 influx permeability coefficient for diffusion is (5.66 ± 0.63) × 10-6 cm/s. The kinetic parameters of PGH2 transport by PGT are Km = 376 ± 34 nM and Vmax = 210.2 ± 11.4 fmol/mg protein/s. PGH2 transport by PGT can be inhibited by excess PGE2 or by a PGT inhibitor. We conclude that PGT may play a role in transporting PGH2 across cellular membranes.

KW - Kinetics

KW - Prostaglandin H

KW - Prostaglandin transporter

UR - http://www.scopus.com/inward/record.url?scp=77951620812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951620812&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2010.03.108

DO - 10.1016/j.bbrc.2010.03.108

M3 - Article

VL - 395

SP - 168

EP - 172

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -