The products of the mdrla and mdrlb genes from multidrug resistant murine cells have similar degradation rates

Dalia Cohen, Huang Yang Chia-Ping Huang Yang, Susan Band Horwitz

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Two vinblastine-resistant sublines of the murine macrophage-like cell line J774.2, J7.V1-1 and J7.V3-1, overproduce unique forms of P-glycoprotein that are encoded by distinct mdr genes, mdrlb and mdrla, respectively. Degradation rates of the two P-glycoprotein isoforms were measured by immunoprecipitation of P-glycoprotein. The half-life of immunoprecipitable P-glycoprotein from J7.V1-1 cells was 16.8 ± 0.5 hours and from J7.V3-1 cells, 17.4 ± 0.5 hours. This rate was not influenced by the presence of vinblastine in the growth medium. The data indicate that P-glycoproteins derived from distinct genes have similar degradation rates.

Original languageEnglish (US)
Pages (from-to)489-495
Number of pages7
JournalLife Sciences
Volume46
Issue number7
DOIs
StatePublished - 1990

Fingerprint

P-Glycoprotein
Genes
Degradation
Vinblastine
P-Glycoproteins
Macrophages
Immunoprecipitation
Half-Life
Protein Isoforms
Cells
Cell Line
Growth

ASJC Scopus subject areas

  • Pharmacology

Cite this

The products of the mdrla and mdrlb genes from multidrug resistant murine cells have similar degradation rates. / Cohen, Dalia; Chia-Ping Huang Yang, Huang Yang; Band Horwitz, Susan.

In: Life Sciences, Vol. 46, No. 7, 1990, p. 489-495.

Research output: Contribution to journalArticle

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