The products of the mdrla and mdrlb genes from multidrug resistant murine cells have similar degradation rates

Dalia Cohen; Huang Yang Chia-Ping Huang Yang; Susan Band Horwitz

doi:10.1016/0024-3205(90)90004-B

The products of the mdrla and mdrlb genes from multidrug resistant murine cells have similar degradation rates

Dalia Cohen, Huang Yang Chia-Ping Huang Yang, Susan Band Horwitz

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Two vinblastine-resistant sublines of the murine macrophage-like cell line J774.2, J7.V1-1 and J7.V3-1, overproduce unique forms of P-glycoprotein that are encoded by distinct mdr genes, mdrlb and mdrla, respectively. Degradation rates of the two P-glycoprotein isoforms were measured by immunoprecipitation of P-glycoprotein. The half-life of immunoprecipitable P-glycoprotein from J7.V1-1 cells was 16.8 ± 0.5 hours and from J7.V3-1 cells, 17.4 ± 0.5 hours. This rate was not influenced by the presence of vinblastine in the growth medium. The data indicate that P-glycoproteins derived from distinct genes have similar degradation rates.

Original language	English (US)
Pages (from-to)	489-495
Number of pages	7
Journal	Life Sciences
Volume	46
Issue number	7
DOIs	https://doi.org/10.1016/0024-3205(90)90004-B
State	Published - 1990

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(90)90004-B

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@article{6db68dc53c1640c2baef01877c0cf445,

title = "The products of the mdrla and mdrlb genes from multidrug resistant murine cells have similar degradation rates",

abstract = "Two vinblastine-resistant sublines of the murine macrophage-like cell line J774.2, J7.V1-1 and J7.V3-1, overproduce unique forms of P-glycoprotein that are encoded by distinct mdr genes, mdrlb and mdrla, respectively. Degradation rates of the two P-glycoprotein isoforms were measured by immunoprecipitation of P-glycoprotein. The half-life of immunoprecipitable P-glycoprotein from J7.V1-1 cells was 16.8 ± 0.5 hours and from J7.V3-1 cells, 17.4 ± 0.5 hours. This rate was not influenced by the presence of vinblastine in the growth medium. The data indicate that P-glycoproteins derived from distinct genes have similar degradation rates.",

author = "Dalia Cohen and {Chia-Ping Huang Yang}, {Huang Yang} and Horwitz, {Susan Band}",

note = "Funding Information: We thank L. Greenberger and S. Hsu for helpful discussions. This work was supported, in part, by United States Public Health Service Grant CA 39821, and a Bristol-Myers Drug Resistance Grant.",

year = "1990",

doi = "10.1016/0024-3205(90)90004-B",

language = "English (US)",

volume = "46",

pages = "489--495",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

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T1 - The products of the mdrla and mdrlb genes from multidrug resistant murine cells have similar degradation rates

AU - Cohen, Dalia

AU - Chia-Ping Huang Yang, Huang Yang

AU - Horwitz, Susan Band

N1 - Funding Information: We thank L. Greenberger and S. Hsu for helpful discussions. This work was supported, in part, by United States Public Health Service Grant CA 39821, and a Bristol-Myers Drug Resistance Grant.

PY - 1990

Y1 - 1990

N2 - Two vinblastine-resistant sublines of the murine macrophage-like cell line J774.2, J7.V1-1 and J7.V3-1, overproduce unique forms of P-glycoprotein that are encoded by distinct mdr genes, mdrlb and mdrla, respectively. Degradation rates of the two P-glycoprotein isoforms were measured by immunoprecipitation of P-glycoprotein. The half-life of immunoprecipitable P-glycoprotein from J7.V1-1 cells was 16.8 ± 0.5 hours and from J7.V3-1 cells, 17.4 ± 0.5 hours. This rate was not influenced by the presence of vinblastine in the growth medium. The data indicate that P-glycoproteins derived from distinct genes have similar degradation rates.

AB - Two vinblastine-resistant sublines of the murine macrophage-like cell line J774.2, J7.V1-1 and J7.V3-1, overproduce unique forms of P-glycoprotein that are encoded by distinct mdr genes, mdrlb and mdrla, respectively. Degradation rates of the two P-glycoprotein isoforms were measured by immunoprecipitation of P-glycoprotein. The half-life of immunoprecipitable P-glycoprotein from J7.V1-1 cells was 16.8 ± 0.5 hours and from J7.V3-1 cells, 17.4 ± 0.5 hours. This rate was not influenced by the presence of vinblastine in the growth medium. The data indicate that P-glycoproteins derived from distinct genes have similar degradation rates.

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The products of the mdrla and mdrlb genes from multidrug resistant murine cells have similar degradation rates

Abstract

ASJC Scopus subject areas

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