The primary event in vision investigated by time-resolved fluorescence spectroscopy

A. G. Doukas; M. R. Junnarkar; R. R. Alfano; R. H. Callender; V. Balogh-Nair

doi:10.1016/S0006-3495(85)83983-7

The primary event in vision investigated by time-resolved fluorescence spectroscopy

A. G. Doukas, M. R. Junnarkar, R. R. Alfano, R. H. Callender, V. Balogh-Nair

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The picosecond fluorescence kinetics and quantum yield from bovine rhodopsin were measured in the 5–40 degrees K range. The fluorescence rise and decay times are faster than our resolution of 15 ps (full width at half maximum) over this entire temperature range. The size of the observed emission was also temperature independent, and we find that the upper limit of rhodopsin's fluorescence quantum yield to be phi f approximately equal to 10(-5). Replacing all of rhodopsin's exchangeable protons with deuterons by suspending rhodopsin in D2O had no effect on either the kinetics of the emission or the value of the quantum yield. Our data provide strong confirmation of the idea that the first step in the visual process is an excited-state cis-to-trans isomerization about the C11-C12 double bond of retinal.

Original language	English (US)
Pages (from-to)	795-798
Number of pages	4
Journal	Biophysical journal
Volume	47
Issue number	6
DOIs	https://doi.org/10.1016/S0006-3495(85)83983-7
State	Published - 1985
Externally published	Yes

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(85)83983-7

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@article{c57c132258864ddab58513d070157433,

title = "The primary event in vision investigated by time-resolved fluorescence spectroscopy",

abstract = "The picosecond fluorescence kinetics and quantum yield from bovine rhodopsin were measured in the 5–40 degrees K range. The fluorescence rise and decay times are faster than our resolution of 15 ps (full width at half maximum) over this entire temperature range. The size of the observed emission was also temperature independent, and we find that the upper limit of rhodopsin's fluorescence quantum yield to be phi f approximately equal to 10(-5). Replacing all of rhodopsin's exchangeable protons with deuterons by suspending rhodopsin in D2O had no effect on either the kinetics of the emission or the value of the quantum yield. Our data provide strong confirmation of the idea that the first step in the visual process is an excited-state cis-to-trans isomerization about the C11-C12 double bond of retinal.",

author = "Doukas, {A. G.} and Junnarkar, {M. R.} and Alfano, {R. R.} and Callender, {R. H.} and V. Balogh-Nair",

note = "Funding Information: This work was supported by National Institutes of Health (NIH) grants EY02515 and EY03142 to City College and NIH grant EY01253 to Columbia University and by a City University Faculty Research Award Program (BPSC-BHE) grant.",

year = "1985",

doi = "10.1016/S0006-3495(85)83983-7",

language = "English (US)",

volume = "47",

pages = "795--798",

journal = "Biophysical journal",

issn = "0006-3495",

publisher = "Biophysical Society",

number = "6",

}

TY - JOUR

T1 - The primary event in vision investigated by time-resolved fluorescence spectroscopy

AU - Doukas, A. G.

AU - Junnarkar, M. R.

AU - Alfano, R. R.

AU - Callender, R. H.

AU - Balogh-Nair, V.

N1 - Funding Information: This work was supported by National Institutes of Health (NIH) grants EY02515 and EY03142 to City College and NIH grant EY01253 to Columbia University and by a City University Faculty Research Award Program (BPSC-BHE) grant.

PY - 1985

Y1 - 1985

N2 - The picosecond fluorescence kinetics and quantum yield from bovine rhodopsin were measured in the 5–40 degrees K range. The fluorescence rise and decay times are faster than our resolution of 15 ps (full width at half maximum) over this entire temperature range. The size of the observed emission was also temperature independent, and we find that the upper limit of rhodopsin's fluorescence quantum yield to be phi f approximately equal to 10(-5). Replacing all of rhodopsin's exchangeable protons with deuterons by suspending rhodopsin in D2O had no effect on either the kinetics of the emission or the value of the quantum yield. Our data provide strong confirmation of the idea that the first step in the visual process is an excited-state cis-to-trans isomerization about the C11-C12 double bond of retinal.

AB - The picosecond fluorescence kinetics and quantum yield from bovine rhodopsin were measured in the 5–40 degrees K range. The fluorescence rise and decay times are faster than our resolution of 15 ps (full width at half maximum) over this entire temperature range. The size of the observed emission was also temperature independent, and we find that the upper limit of rhodopsin's fluorescence quantum yield to be phi f approximately equal to 10(-5). Replacing all of rhodopsin's exchangeable protons with deuterons by suspending rhodopsin in D2O had no effect on either the kinetics of the emission or the value of the quantum yield. Our data provide strong confirmation of the idea that the first step in the visual process is an excited-state cis-to-trans isomerization about the C11-C12 double bond of retinal.

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U2 - 10.1016/S0006-3495(85)83983-7

DO - 10.1016/S0006-3495(85)83983-7

M3 - Article

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AN - SCOPUS:0022083010

SN - 0006-3495

VL - 47

SP - 795

EP - 798

JO - Biophysical journal

JF - Biophysical journal

IS - 6

ER -

The primary event in vision investigated by time-resolved fluorescence spectroscopy

Abstract

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