The potential role of lung microbiota in lung cancer attributed to household coal burning exposures

H. Dean Hosgood, Amy R. Sapkota, Nathaniel Rothman, Thomas Rohan, Wei Hu, Jun Xu, Roel Vermeulen, Xingzhou He, James Robert White, Guoping Wu, Fusheng Wei, Emmanuel F. Mongodin, Qing Lan

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Bacteria influence site-specific disease etiology and the host's ability to metabolize xenobiotics, such as polycyclic aromatic hydrocarbons (PAHs). Lung cancer in Xuanwei, China has been attributed to PAH-rich household air pollution from burning coal. This study seeks to explore the role of lung microbiota in lung cancer among never smoking Xuanwei women and how coal burning may influence these associations. DNA from sputum and buccal samples of never smoking lung cancer cases (n=8, in duplicate) and controls (n=8, in duplicate) in two Xuanwei villages was extracted using a multi-step enzymatic and physical lysis, followed by a standardized clean-up. V1-V2 regions of 16S rRNA genes were PCR-amplified. Purified amplicons were sequenced by 454 FLX Titanium pyrosequencing and high-quality sequences were evaluated for diversity and taxonomic membership. Bacterial diversity among cases and controls was similar in buccal samples (P=0.46), but significantly different in sputum samples (P=0.038). In sputum, Granulicatella (6.1 vs. 2.0%; P=0.0016), Abiotrophia (1.5 vs. 0.085%; P=0.0036), and Streptococcus (40.1 vs. 19.8%; P=0.0142) were enriched in cases compared with controls. Sputum samples had on average 488.25 species-level OTUs in the flora of cases who used smoky coal (PAH-rich) compared with 352.5 OTUs among cases who used smokeless coal (PAH-poor; P=0.047). These differences were explained by the Bacilli species (Streptococcus infantis and Streptococcus anginosus). Our small study suggests that never smoking lung cancer cases have differing sputum microbiota than controls. Further, bacteria found in sputum may be influenced by environmental exposures associated with the type of coal burned in the home.

Original languageEnglish (US)
Pages (from-to)643-651
Number of pages9
JournalEnvironmental and Molecular Mutagenesis
Volume55
Issue number8
DOIs
StatePublished - Oct 1 2014

Keywords

  • 16S
  • Bacteria
  • Carcinogenesis
  • Lung cancer
  • Lung microbiota
  • Pulmonary
  • Respiratory

ASJC Scopus subject areas

  • Epidemiology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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  • Cite this

    Hosgood, H. D., Sapkota, A. R., Rothman, N., Rohan, T., Hu, W., Xu, J., Vermeulen, R., He, X., White, J. R., Wu, G., Wei, F., Mongodin, E. F., & Lan, Q. (2014). The potential role of lung microbiota in lung cancer attributed to household coal burning exposures. Environmental and Molecular Mutagenesis, 55(8), 643-651. https://doi.org/10.1002/em.21878