The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12

Jacob V. Maizel, David O. White, Matthew D. Scharff

Research output: Contribution to journalArticle

564 Citations (Scopus)

Abstract

The adenovirion has been shown to contain at least nine different polypeptides demonstrable by dissociation and acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Each polypeptide of adenovirus type 2 is chemically distinct by isotopic ratio analysis, and they all contain lysine, arginine, tryptophan, valine, and threonine. The molecular weights of these polypeptides determined by gel electrophoresis range from 120,000 for the largest and most prominent component to 7500 for the smallest. Comparison of nontumorigenic type 2 with tumorigenic types 7A and 12 by double-isotope labeling revealed a generally similar peptide pattern for all types. However, there were distinct differences between the corresponding peptides of all three types. These results imply extensive differences in the genes for most of the capsid proteins.

Original languageEnglish (US)
Pages (from-to)115-125
Number of pages11
JournalVirology
Volume36
Issue number1
StatePublished - Sep 1968

Fingerprint

Adenoviridae
Virion
Peptides
Proteins
Electrophoresis
Gels
Isotope Labeling
Acrylamide
Valine
Capsid Proteins
Threonine
Tryptophan
Sodium Dodecyl Sulfate
Lysine
Arginine
Molecular Weight
Genes

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. / Maizel, Jacob V.; White, David O.; Scharff, Matthew D.

In: Virology, Vol. 36, No. 1, 09.1968, p. 115-125.

Research output: Contribution to journalArticle

@article{1bbc27d8dcf149c89a158e89c2611336,
title = "The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12",
abstract = "The adenovirion has been shown to contain at least nine different polypeptides demonstrable by dissociation and acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Each polypeptide of adenovirus type 2 is chemically distinct by isotopic ratio analysis, and they all contain lysine, arginine, tryptophan, valine, and threonine. The molecular weights of these polypeptides determined by gel electrophoresis range from 120,000 for the largest and most prominent component to 7500 for the smallest. Comparison of nontumorigenic type 2 with tumorigenic types 7A and 12 by double-isotope labeling revealed a generally similar peptide pattern for all types. However, there were distinct differences between the corresponding peptides of all three types. These results imply extensive differences in the genes for most of the capsid proteins.",
author = "Maizel, {Jacob V.} and White, {David O.} and Scharff, {Matthew D.}",
year = "1968",
month = "9",
language = "English (US)",
volume = "36",
pages = "115--125",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12

AU - Maizel, Jacob V.

AU - White, David O.

AU - Scharff, Matthew D.

PY - 1968/9

Y1 - 1968/9

N2 - The adenovirion has been shown to contain at least nine different polypeptides demonstrable by dissociation and acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Each polypeptide of adenovirus type 2 is chemically distinct by isotopic ratio analysis, and they all contain lysine, arginine, tryptophan, valine, and threonine. The molecular weights of these polypeptides determined by gel electrophoresis range from 120,000 for the largest and most prominent component to 7500 for the smallest. Comparison of nontumorigenic type 2 with tumorigenic types 7A and 12 by double-isotope labeling revealed a generally similar peptide pattern for all types. However, there were distinct differences between the corresponding peptides of all three types. These results imply extensive differences in the genes for most of the capsid proteins.

AB - The adenovirion has been shown to contain at least nine different polypeptides demonstrable by dissociation and acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Each polypeptide of adenovirus type 2 is chemically distinct by isotopic ratio analysis, and they all contain lysine, arginine, tryptophan, valine, and threonine. The molecular weights of these polypeptides determined by gel electrophoresis range from 120,000 for the largest and most prominent component to 7500 for the smallest. Comparison of nontumorigenic type 2 with tumorigenic types 7A and 12 by double-isotope labeling revealed a generally similar peptide pattern for all types. However, there were distinct differences between the corresponding peptides of all three types. These results imply extensive differences in the genes for most of the capsid proteins.

UR - http://www.scopus.com/inward/record.url?scp=0014329310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014329310&partnerID=8YFLogxK

M3 - Article

C2 - 5669982

AN - SCOPUS:0014329310

VL - 36

SP - 115

EP - 125

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -