The place of inactivated actin and its kinetic predecessor in actin folding - Unfolding

Irina M. Kuznetsova, Olga V. Stepanenko, Olesia V. Stepanenko, Olga I. Povarova, Alexander G. Biktashev, Vladislav Verkhusha, Mikhail M. Shavlovsky, Konstantin K. Turoverov

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The kinetics of actin unfolding induced by guanidine hydrochloride of different concentrations was studied. The parametric representation of the kinetic dependencies of tryptophan fluorescence intensity changes recorded at two wavelengths allowed us to detect and characterize a new essentially unfolded kinetic intermediate. Its characteristics suggested that this intermediate state is a premolten globule. It was shown that the equilibrium transition between inactivated and completely unfolded states is also a two-step process and proceeds via an essentially unfolded kinetic intermediate. The new kinetic pathway of actin unfolding - refolding was proposed. According to it, the founded essentially unfolded kinetic state is the on-pathway intermediate, while inactivated actin is the off-pathway misfolded state stabilized by aggregation of partially folded macromolecules of protein.

Original languageEnglish (US)
Pages (from-to)13127-13132
Number of pages6
JournalBiochemistry
Volume41
Issue number44
DOIs
StatePublished - Nov 5 2002
Externally publishedYes

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Actins
Kinetics
Guanidine
Macromolecules
Tryptophan
Agglomeration
Fluorescence
Wavelength
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kuznetsova, I. M., Stepanenko, O. V., Stepanenko, O. V., Povarova, O. I., Biktashev, A. G., Verkhusha, V., ... Turoverov, K. K. (2002). The place of inactivated actin and its kinetic predecessor in actin folding - Unfolding. Biochemistry, 41(44), 13127-13132. https://doi.org/10.1021/bi026412x

The place of inactivated actin and its kinetic predecessor in actin folding - Unfolding. / Kuznetsova, Irina M.; Stepanenko, Olga V.; Stepanenko, Olesia V.; Povarova, Olga I.; Biktashev, Alexander G.; Verkhusha, Vladislav; Shavlovsky, Mikhail M.; Turoverov, Konstantin K.

In: Biochemistry, Vol. 41, No. 44, 05.11.2002, p. 13127-13132.

Research output: Contribution to journalArticle

Kuznetsova, IM, Stepanenko, OV, Stepanenko, OV, Povarova, OI, Biktashev, AG, Verkhusha, V, Shavlovsky, MM & Turoverov, KK 2002, 'The place of inactivated actin and its kinetic predecessor in actin folding - Unfolding', Biochemistry, vol. 41, no. 44, pp. 13127-13132. https://doi.org/10.1021/bi026412x
Kuznetsova IM, Stepanenko OV, Stepanenko OV, Povarova OI, Biktashev AG, Verkhusha V et al. The place of inactivated actin and its kinetic predecessor in actin folding - Unfolding. Biochemistry. 2002 Nov 5;41(44):13127-13132. https://doi.org/10.1021/bi026412x
Kuznetsova, Irina M. ; Stepanenko, Olga V. ; Stepanenko, Olesia V. ; Povarova, Olga I. ; Biktashev, Alexander G. ; Verkhusha, Vladislav ; Shavlovsky, Mikhail M. ; Turoverov, Konstantin K. / The place of inactivated actin and its kinetic predecessor in actin folding - Unfolding. In: Biochemistry. 2002 ; Vol. 41, No. 44. pp. 13127-13132.
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