The PGE2/IL-10 axis determines susceptibility of B-1 Cell-Derived Phagocytes (B-1CDP) to Leishmania major infection

Angélica F. Arcanjo, Isabel F. LaRocque-de-Freitas, Juliana Dutra B. Rocha, Daniel Zamith, Ana Caroline Costa-da-Silva, Marise Pinheiro Nunes, Fabio P. Mesquita-Santos, Alexandre Morrot, Alessandra A. Filardy, Mario Mariano, Christianne Bandeira-Melo, George A. DosReis, Debora Decote-Ricardo, Célio Geraldo Freire-de-Lima

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

B-1 cells can be differentiated from B-2 cells because they are predominantly located in the peritoneal and pleural cavities and have distinct phenotypic patterns and activation properties. A mononuclear phagocyte derived from B-1 cells (B-1CDP) has been described. As the B-1CDP cells migrate to inflammatory/infectious sites and exhibit phagocytic capacity, the microbicidal ability of these cells was investigated using the Leishmania major infection model in vitro. The data obtained in this study demonstrate that B-1CDP cells are more susceptible to infection than peritoneal macrophages, since B-1CDP cells have a higher number of intracellular amastigotes forms and consequently release a larger number of promastigotes. Exacerbated infection by L. major required lipid bodies/PGE2 and IL-10 by B-1CDP cells. Both infection and the production of IL-10 were decreased when PGE2 production was blocked by NSAIDs. The involvement of IL-10 in this mechanism was confirmed, since B-1CDP cells from IL-10 KO mice are more competent to control L. major infection than cells from wild type mice. These findings further characterize the B-1CDP cells as an important mononuclear phagocyte that plays a previously unrecognized role in host responses to L. major infection, most likely via PGE2-driven production of IL-10.

Original languageEnglish (US)
Article numbere0124888
JournalPloS one
Volume10
Issue number5
DOIs
StatePublished - May 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'The PGE<sub>2</sub>/IL-10 axis determines susceptibility of B-1 Cell-Derived Phagocytes (B-1CDP) to Leishmania major infection'. Together they form a unique fingerprint.

Cite this