The percentage of dense red cells does not predict incidence of sickle cell painful crisis

To test the hypothesis that the tendency of hemoglobin S (Hbs) to polymerize within cells is the major determinant of the incidence of vaso-occlusive episodes, we have examined the effect of the percentage of dense cells (as measured by Percoll-Stractan continuous density gradient centrifugation) on the frequency of painful crises in a group of 36 patients with sickle cell disease. No correlation was found between the percentage of dense cells and admissions for crisis. Among the patients with known α-gene status (n=25), the strong correlation between decreased dense cells and α-thalassemia (-α/αα) reported previously was confirmed (P<.001). In addition, in this small subset, patients with α-thalassemia (-α/αα) appeared to have a marginally increased number of admissions for sickle cell crisis (t=2.1910, P<.05), which was independent of the percentage of dense cells. We conclude that the percentage of dense sickle cells cannot predict the incidence of painful crisis, suggesting that other factors (microcirculatory regulation or other humoral and cellular factors) are more important in the generation maintenance of painful crises than the necessary, but not sufficient, tendency of HbS-containing red cells to sickle.