The pathogenicity island gene irp-2 of Yersinia enterocolitica was detected in enteric Shiga-like-toxin-producing and invasive E. coli isolated in China

Bokun Cheng, Changyun Ye, Jianhong Wu

Research output: Contribution to journalArticle

Abstract

Objective: Detection of the pathogenicity island gene irp-2 of Yersinia enterocolitica species from enteric Shiga-like-toxin-producing and invasive Escherichia coli (ESIEC) strains isolated in various provinces of China. Methods: Colony hybridization, DNA dot hybridization and polymerase chain reaction (PCR). Results: By colony blots, 67 of the 371 (18.06%) strains of ESIEC were hybridized with the irp-2 gene probe. The replicon of irp-2 gene was identified by PCR method in 64 of 67 strains (95 5%) hybridized with irp-2 probe. And, one of the 67 strains hybridized with ipaB gene probe of entero-invasive E. coli (EIEC). A few strains of EIEC have been proved to be irp-2 gene positive. 20 irp-2-positive and 20 irp-2-negative strains of ESIEC, demonstrated by colony hybridization and PCR methods were selected to test irp-2 and ipaB genes by DNA dot blots. 38 of 40 strains were shown to be identical. Conclusion: The irp-2 of Yersinia species is widely existed among the ESIEC strains isolated in China, and the irp-2+ ipaB- could be used as one of the diagnostic marker for some strain that were named ESIEC before.

Original languageEnglish (US)
Pages (from-to)489-492
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume20
Issue number6
StatePublished - Nov 1 2000
Externally publishedYes

Fingerprint

Shiga Toxins
Genomic Islands
Yersinia enterocolitica
China
Escherichia coli
Genes
Polymerase Chain Reaction
Yersinia
Replicon
DNA

Keywords

  • ESIEC
  • Hybridization
  • irp-2 gene
  • PCR

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

@article{411bb0817aaa4770b0efa9a84f4c1542,
title = "The pathogenicity island gene irp-2 of Yersinia enterocolitica was detected in enteric Shiga-like-toxin-producing and invasive E. coli isolated in China",
abstract = "Objective: Detection of the pathogenicity island gene irp-2 of Yersinia enterocolitica species from enteric Shiga-like-toxin-producing and invasive Escherichia coli (ESIEC) strains isolated in various provinces of China. Methods: Colony hybridization, DNA dot hybridization and polymerase chain reaction (PCR). Results: By colony blots, 67 of the 371 (18.06{\%}) strains of ESIEC were hybridized with the irp-2 gene probe. The replicon of irp-2 gene was identified by PCR method in 64 of 67 strains (95 5{\%}) hybridized with irp-2 probe. And, one of the 67 strains hybridized with ipaB gene probe of entero-invasive E. coli (EIEC). A few strains of EIEC have been proved to be irp-2 gene positive. 20 irp-2-positive and 20 irp-2-negative strains of ESIEC, demonstrated by colony hybridization and PCR methods were selected to test irp-2 and ipaB genes by DNA dot blots. 38 of 40 strains were shown to be identical. Conclusion: The irp-2 of Yersinia species is widely existed among the ESIEC strains isolated in China, and the irp-2+ ipaB- could be used as one of the diagnostic marker for some strain that were named ESIEC before.",
keywords = "ESIEC, Hybridization, irp-2 gene, PCR",
author = "Bokun Cheng and Changyun Ye and Jianhong Wu",
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language = "English (US)",
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journal = "Chinese Journal of Microbiology and Immunology",
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TY - JOUR

T1 - The pathogenicity island gene irp-2 of Yersinia enterocolitica was detected in enteric Shiga-like-toxin-producing and invasive E. coli isolated in China

AU - Cheng, Bokun

AU - Ye, Changyun

AU - Wu, Jianhong

PY - 2000/11/1

Y1 - 2000/11/1

N2 - Objective: Detection of the pathogenicity island gene irp-2 of Yersinia enterocolitica species from enteric Shiga-like-toxin-producing and invasive Escherichia coli (ESIEC) strains isolated in various provinces of China. Methods: Colony hybridization, DNA dot hybridization and polymerase chain reaction (PCR). Results: By colony blots, 67 of the 371 (18.06%) strains of ESIEC were hybridized with the irp-2 gene probe. The replicon of irp-2 gene was identified by PCR method in 64 of 67 strains (95 5%) hybridized with irp-2 probe. And, one of the 67 strains hybridized with ipaB gene probe of entero-invasive E. coli (EIEC). A few strains of EIEC have been proved to be irp-2 gene positive. 20 irp-2-positive and 20 irp-2-negative strains of ESIEC, demonstrated by colony hybridization and PCR methods were selected to test irp-2 and ipaB genes by DNA dot blots. 38 of 40 strains were shown to be identical. Conclusion: The irp-2 of Yersinia species is widely existed among the ESIEC strains isolated in China, and the irp-2+ ipaB- could be used as one of the diagnostic marker for some strain that were named ESIEC before.

AB - Objective: Detection of the pathogenicity island gene irp-2 of Yersinia enterocolitica species from enteric Shiga-like-toxin-producing and invasive Escherichia coli (ESIEC) strains isolated in various provinces of China. Methods: Colony hybridization, DNA dot hybridization and polymerase chain reaction (PCR). Results: By colony blots, 67 of the 371 (18.06%) strains of ESIEC were hybridized with the irp-2 gene probe. The replicon of irp-2 gene was identified by PCR method in 64 of 67 strains (95 5%) hybridized with irp-2 probe. And, one of the 67 strains hybridized with ipaB gene probe of entero-invasive E. coli (EIEC). A few strains of EIEC have been proved to be irp-2 gene positive. 20 irp-2-positive and 20 irp-2-negative strains of ESIEC, demonstrated by colony hybridization and PCR methods were selected to test irp-2 and ipaB genes by DNA dot blots. 38 of 40 strains were shown to be identical. Conclusion: The irp-2 of Yersinia species is widely existed among the ESIEC strains isolated in China, and the irp-2+ ipaB- could be used as one of the diagnostic marker for some strain that were named ESIEC before.

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KW - Hybridization

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KW - PCR

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