TY - JOUR
T1 - The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression
AU - Zhou, Jie
AU - Ashouian, Nasrin
AU - Delepine, Marc
AU - Matsuda, Fumihiko
AU - Chevillard, Christophe
AU - Riblet, Roy
AU - Schildkraut, Carl L.
AU - Birshtein, Barbara K.
PY - 2002/10/15
Y1 - 2002/10/15
N2 - The 3′ Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes - Crip, Crp2, and Mta1 - are located ≊70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3′ Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.
AB - The 3′ Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes - Crip, Crp2, and Mta1 - are located ≊70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3′ Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.
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U2 - 10.1073/pnas.212392399
DO - 10.1073/pnas.212392399
M3 - Article
C2 - 12370427
AN - SCOPUS:0037108950
SN - 0027-8424
VL - 99
SP - 13693
EP - 13698
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 21
ER -