The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression

Jie Zhou, Nasrin Ashouian, Marc Delepine, Fumihiko Matsuda, Christophe Chevillard, Roy Riblet, Carl L. Schildkraut, Barbara K. Birshtein

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Abstract

The 3′ Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes - Crip, Crp2, and Mta1 - are located ≊70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3′ Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.

Original languageEnglish (US)
Pages (from-to)13693-13698
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number21
DOIs
Publication statusPublished - Oct 15 2002

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