The origin and development of nonlymphoid tissue CD103+ DCs

Florent Ginhoux, Kang Liu, Julie Helft, Milena Bogunovic, Melanie Greter, Daigo Hashimoto, Jeremy Price, Na Yin, Jonathan Bromberg, Sergio A. Lira, E. Richard Stanley, Michel Nussenzweig, Miriam Merad

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494 Scopus citations

Abstract

CD103+ dendritic cells (DCs) in nonlymphoid tissues are specialized in the cross-presentation of cell-associated antigens. However, little is known about the mechanisms that regulate the development of these cells. We show that two populations of CD11c+MHCII+ cells separated on the basis of CD103 and CD11b expression coexist in most nonlymphoid tissues with the exception of the lamina propria. CD103+ DCs are related to lymphoid organ CD8+ DCs in that they are derived exclusively from pre-DCs under the control of fms-like tyrosine kinase 3 (Flt3) ligand, inhibitor of DNA protein 2 (Id2), and IFN regulatory protein 8 (IRF8). In contrast, lamina propria CD103+ DCs express CD11b and develop independently of Id2 and IRF8. The other population of CD11c +MHCII+ cells in tissues, which is CD103-CD11b +, is heterogenous and depends on both Flt3 and MCSF-R. Our results reveal that nonlymphoid tissue CD103+ DCs and lymphoid organ CD8 + DCs derive from the same precursor and follow a related differentiation program.

Original languageEnglish (US)
Pages (from-to)3115-3130
Number of pages16
JournalJournal of Experimental Medicine
Volume206
Issue number13
DOIs
StatePublished - Dec 21 2009

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ginhoux, F., Liu, K., Helft, J., Bogunovic, M., Greter, M., Hashimoto, D., Price, J., Yin, N., Bromberg, J., Lira, S. A., Stanley, E. R., Nussenzweig, M., & Merad, M. (2009). The origin and development of nonlymphoid tissue CD103+ DCs. Journal of Experimental Medicine, 206(13), 3115-3130. https://doi.org/10.1084/jem.20091756