TY - JOUR
T1 - THE ORGANIZATION OF ASTROCYTES IN ORGANOTYPIC MOUSE SPINAL CORD CULTURE
T2 - AN ELECTRON MICROSCOPE STUDY
AU - KUSAKA, H.
AU - HIRANO, A.
AU - BORNSTEIN, M. B.
AU - RAINE, C. S.
PY - 1984/11
Y1 - 1984/11
N2 - The organization of astrocytes in organotypic mouse spinal cord culture: an electron microscope study The organization of astrocytes in myelinated culture of mouse spinal cord tissue was analysed ultrastructurally and compared with the pattern in vivo. Astrocyte cell bodies and their processes, connected by punctate adhesions and gap junctions, formed a continuous layer around the entire explant. Throughout the parenchyma, thin astrocytic processes penetrated the neuropil, separated neurons, and invested some synaptic complexes. Others formed flattened sheets and contacted directly with the basal surface of ependymal cells. In the absence of mesenchymal elements, astrocytes in vitro occasionally possessed fragments of basal lamina on the surface of the explant and around minute intercellular spaces. Except for an incomplete basal lamina, these findings indicate that astrocytes in vitro are organized in a manner essentially identical to the situation in vivo.
AB - The organization of astrocytes in organotypic mouse spinal cord culture: an electron microscope study The organization of astrocytes in myelinated culture of mouse spinal cord tissue was analysed ultrastructurally and compared with the pattern in vivo. Astrocyte cell bodies and their processes, connected by punctate adhesions and gap junctions, formed a continuous layer around the entire explant. Throughout the parenchyma, thin astrocytic processes penetrated the neuropil, separated neurons, and invested some synaptic complexes. Others formed flattened sheets and contacted directly with the basal surface of ependymal cells. In the absence of mesenchymal elements, astrocytes in vitro occasionally possessed fragments of basal lamina on the surface of the explant and around minute intercellular spaces. Except for an incomplete basal lamina, these findings indicate that astrocytes in vitro are organized in a manner essentially identical to the situation in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0021675662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021675662&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2990.1984.tb00390.x
DO - 10.1111/j.1365-2990.1984.tb00390.x
M3 - Article
C2 - 6527730
AN - SCOPUS:0021675662
SN - 0305-1846
VL - 10
SP - 411
EP - 422
JO - Neuropathology and Applied Neurobiology
JF - Neuropathology and Applied Neurobiology
IS - 6
ER -