The octarepeat domain of the prion protein binds Cu(II) with three distinct coordination modes at pH 7.4

Madhuri Chattopadhyay, Eric D. Walter, Dustin J. Newell, Pilgrim J. Jackson, Eliah Aronoff-Spencer, Jack Peisach, Gary J. Gerfen, Brian Bennett, William E. Antholine, Glenn L. Millhauser

Research output: Contribution to journalArticle

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Abstract

The prion protein (PrP) binds Cu2+ in its N-terminal octarepeat domain. This unusual domain is comprised of four or more tandem repeats of the fundamental sequence PHGGGWGQ. Previous work from our laboratories demonstrates that at full copper occupancy, each HGGGW segment binds a single Cu 2+. However, several recent studies suggest that low copper occupancy favors different coordination modes, possibly involving imidazoles from histidines in adjacent octapeptide segments. This is investigated here using a combination of X-band EPR, S-band EPR, and ESEEM, along with a library of modified peptides designed to favor different coordination interactions. At pH 7.4, three distinct coordination modes are identified. Each mode is fully characterized to reveal a series of copper-dependent octarepeat domain structures. Multiple His coordination is clearly identified at low copper stoichiometry. In addition, EPR detected copper-copper interactions at full occupancy suggest that the octarepeat domain partially collapses, perhaps stabilizing this specific binding mode and facilitating cooperative copper uptake. This work provides the first complete characterization of all dominant copper coordination modes at pH 7.4.

Original languageEnglish (US)
Pages (from-to)12647-12656
Number of pages10
JournalJournal of the American Chemical Society
Volume127
Issue number36
DOIs
StatePublished - Sep 14 2005

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Copper
Paramagnetic resonance
Imidazoles
Peptide Library
Tandem Repeat Sequences
Prions
Prion Proteins
Histidine
Stoichiometry
Peptides

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Chattopadhyay, M., Walter, E. D., Newell, D. J., Jackson, P. J., Aronoff-Spencer, E., Peisach, J., ... Millhauser, G. L. (2005). The octarepeat domain of the prion protein binds Cu(II) with three distinct coordination modes at pH 7.4. Journal of the American Chemical Society, 127(36), 12647-12656. https://doi.org/10.1021/ja053254z

The octarepeat domain of the prion protein binds Cu(II) with three distinct coordination modes at pH 7.4. / Chattopadhyay, Madhuri; Walter, Eric D.; Newell, Dustin J.; Jackson, Pilgrim J.; Aronoff-Spencer, Eliah; Peisach, Jack; Gerfen, Gary J.; Bennett, Brian; Antholine, William E.; Millhauser, Glenn L.

In: Journal of the American Chemical Society, Vol. 127, No. 36, 14.09.2005, p. 12647-12656.

Research output: Contribution to journalArticle

Chattopadhyay, M, Walter, ED, Newell, DJ, Jackson, PJ, Aronoff-Spencer, E, Peisach, J, Gerfen, GJ, Bennett, B, Antholine, WE & Millhauser, GL 2005, 'The octarepeat domain of the prion protein binds Cu(II) with three distinct coordination modes at pH 7.4', Journal of the American Chemical Society, vol. 127, no. 36, pp. 12647-12656. https://doi.org/10.1021/ja053254z
Chattopadhyay M, Walter ED, Newell DJ, Jackson PJ, Aronoff-Spencer E, Peisach J et al. The octarepeat domain of the prion protein binds Cu(II) with three distinct coordination modes at pH 7.4. Journal of the American Chemical Society. 2005 Sep 14;127(36):12647-12656. https://doi.org/10.1021/ja053254z
Chattopadhyay, Madhuri ; Walter, Eric D. ; Newell, Dustin J. ; Jackson, Pilgrim J. ; Aronoff-Spencer, Eliah ; Peisach, Jack ; Gerfen, Gary J. ; Bennett, Brian ; Antholine, William E. ; Millhauser, Glenn L. / The octarepeat domain of the prion protein binds Cu(II) with three distinct coordination modes at pH 7.4. In: Journal of the American Chemical Society. 2005 ; Vol. 127, No. 36. pp. 12647-12656.
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