The Oct-2 protein binds cooperatively to adjacent octamer sites.

J. H. LeBowitz, R. G. Clerc, Michael D. Brenowitz, P. A. Sharp

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Recombinant proteins derived from the cloned human oct-2 gene were used to investigate cooperative binding by Oct-2 to adjacent DNA-binding sites. Oct-2, a B-cell-specific transcription factor, binds tightly to the octamer sequence in immunoglobulin promoters. A second apparently unrelated consensus sequence in heavy chain promoters, the heptamer site, also is recognized by the Oct-2 protein but with 1000-fold lower affinity. Simultaneous occupancy of both the octamer and heptamer sites is favored by cooperative interactions. The heptamer site is probably recognized by the same binding surface in the Oct-2 protein as the octamer site and thus is conserved as a lower-affinity binding site. This permits the immunoglobulin promoter to respond to a much broader range of levels of Oct-2 protein. Substitution of prototype octamer sequences for heptamer sequences yields a probe with two octamer sites spaced by 2 nucleotides, which also binds Oct-2 protein cooperatively. Only the POU domain in the Oct-2 protein is required for this cooperative interaction. Similar protein-protein interactions between bound Oct-2 proteins may promote promoter-enhancer synergism in the heavy chain gene.

Original languageEnglish (US)
Pages (from-to)1625-1638
Number of pages14
JournalGenes & development
Volume3
Issue number10
StatePublished - Oct 1989
Externally publishedYes

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Octamer Transcription Factors
Immunoglobulins
PAX5 Transcription Factor
Binding Sites
Consensus Sequence
Recombinant Proteins
Genes
Proteins
Nucleotides
DNA

ASJC Scopus subject areas

  • Developmental Biology

Cite this

LeBowitz, J. H., Clerc, R. G., Brenowitz, M. D., & Sharp, P. A. (1989). The Oct-2 protein binds cooperatively to adjacent octamer sites. Genes & development, 3(10), 1625-1638.

The Oct-2 protein binds cooperatively to adjacent octamer sites. / LeBowitz, J. H.; Clerc, R. G.; Brenowitz, Michael D.; Sharp, P. A.

In: Genes & development, Vol. 3, No. 10, 10.1989, p. 1625-1638.

Research output: Contribution to journalArticle

LeBowitz, JH, Clerc, RG, Brenowitz, MD & Sharp, PA 1989, 'The Oct-2 protein binds cooperatively to adjacent octamer sites.', Genes & development, vol. 3, no. 10, pp. 1625-1638.
LeBowitz, J. H. ; Clerc, R. G. ; Brenowitz, Michael D. ; Sharp, P. A. / The Oct-2 protein binds cooperatively to adjacent octamer sites. In: Genes & development. 1989 ; Vol. 3, No. 10. pp. 1625-1638.
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