The nutritional environment determines which and how intestinal stem cells contribute to homeostasis and tumorigenesis

Wenge Li, Samuel E. Zimmerman, Karina Peregrina, Michele Houston, Joshua Mayoral, Jinghang Zhang, Shahina Maqbool, Zhengdong Zhang, Ying Cai, Kenny Ye, Leonard H. Augenlicht

Research output: Contribution to journalArticle

Abstract

Sporadic colon cancer accounts for approximately 80% of colorectal cancer (CRC) with high incidence in Western societies strongly linked to long-term dietary patterns. A unique mouse model for sporadic CRC results from feeding a purified rodent Western-style diet (NWD1) recapitulating intake for the mouse of common nutrient risk factors each at its level consumed in higher risk Western populations. This causes sporadic large and small intestinal tumors in wild-type mice at an incidence and frequency similar to that in humans. NWD1 perturbs intestinal cell maturation and Wnt signaling throughout villi and colonic crypts and decreases mouse Lgr5hi intestinal stem cell contribution to homeostasis and tumor development. Here we establish that NWD1 transcriptionally reprograms Lgr5hi cells, and that nutrients are interactive in reprogramming. Furthermore, the DNA mismatch repair pathway is elevated in Lgr5hi cells by lower vitamin D3 and/or calcium in NWD1, paralleled by reduced accumulation of relevant somatic mutations detected by single-cell exome sequencing. In compensation, NWD1 also reprograms Bmi1+ cells to function and persist as stem-like cells in mucosal homeostasis and tumor development. The data establish the key role of the nutrient environment in defining the contribution of two different stem cell populations to both mucosal homeostasis and tumorigenesis. This raises important questions regarding impact of variable human diets on which and how stem cell populations function in the human mucosa and give rise to tumors. Moreover, major differences reported in turnover of human and mouse crypt base stem cells may be linked to their very different nutrient exposures.

Original languageEnglish (US)
Pages (from-to)937-946
Number of pages10
JournalCarcinogenesis
Volume40
Issue number8
DOIs
StatePublished - Aug 22 2019

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Carcinogenesis
Homeostasis
Stem Cells
Food
Colorectal Neoplasms
Neoplasms
Population
Diet
Exome
DNA Mismatch Repair
Cholecalciferol
Incidence
Colonic Neoplasms
Rodentia
Mucous Membrane
Calcium
Mutation

ASJC Scopus subject areas

  • Cancer Research

Cite this

The nutritional environment determines which and how intestinal stem cells contribute to homeostasis and tumorigenesis. / Li, Wenge; Zimmerman, Samuel E.; Peregrina, Karina; Houston, Michele; Mayoral, Joshua; Zhang, Jinghang; Maqbool, Shahina; Zhang, Zhengdong; Cai, Ying; Ye, Kenny; Augenlicht, Leonard H.

In: Carcinogenesis, Vol. 40, No. 8, 22.08.2019, p. 937-946.

Research output: Contribution to journalArticle

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AU - Maqbool, Shahina

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