The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox gene expression

H. Zhang, Scott W. Emmons

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We describe the properties of a new gene, sop-3, that is required for the regulated expression of a C. elegans Hox gene, egl-5, in a postembryonic neuroectodermal cell lineage. Regulated expression of egl-5 in this cell lineage is necessary for development of the sensory rays of the male tail. sop-3 encodes a predicted novel protein of 1475 amino acids without clear homologs in other organisms. However, the sequence contains motifs consisting of homopolymeric runs of amino acids found in several other transcriptional regulators, some of which also act in Hox gene regulatory pathways. The genetic properties of sop-3 are very similar to those of sop-1, which encodes a component of the transcriptional Mediator complex, and mutations in the two genes are synthetic lethal. This suggests that SOP-3 may act at the level of the Mediator complex in regulating transcription initiation. In a sop-3 loss-of-function background, egl-5 is expressed ectopically in lineage branches that normally do not express this gene. Such expression is dependent on the Hox gene mab-5, as it is in branches where egl-5 is normally expressed. Ectopic egl-5 expression is also dependent on the Wnt pathway. Thus, sop-3 contributes to the combinatorial control of egl-5 by blocking egl-5 activation by MAB-5 and the Wnt pathway in inappropriate lineage branches.

Original languageEnglish (US)
Pages (from-to)767-777
Number of pages11
JournalDevelopment
Volume128
Issue number5
StatePublished - 2001

Fingerprint

Homeobox Genes
Mediator Complex
Wnt Signaling Pathway
Cell Lineage
Gene Expression
Lethal Genes
Genes
Synthetic Genes
Amino Acids
Mutation
Proteins

Keywords

  • C. elegans
  • Homeobox gene
  • Regulation
  • Sop-3
  • Transcription
  • Wnt signaling

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox gene expression. / Zhang, H.; Emmons, Scott W.

In: Development, Vol. 128, No. 5, 2001, p. 767-777.

Research output: Contribution to journalArticle

@article{2aa7abddf44d49d79394b07aba08a360,
title = "The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox gene expression",
abstract = "We describe the properties of a new gene, sop-3, that is required for the regulated expression of a C. elegans Hox gene, egl-5, in a postembryonic neuroectodermal cell lineage. Regulated expression of egl-5 in this cell lineage is necessary for development of the sensory rays of the male tail. sop-3 encodes a predicted novel protein of 1475 amino acids without clear homologs in other organisms. However, the sequence contains motifs consisting of homopolymeric runs of amino acids found in several other transcriptional regulators, some of which also act in Hox gene regulatory pathways. The genetic properties of sop-3 are very similar to those of sop-1, which encodes a component of the transcriptional Mediator complex, and mutations in the two genes are synthetic lethal. This suggests that SOP-3 may act at the level of the Mediator complex in regulating transcription initiation. In a sop-3 loss-of-function background, egl-5 is expressed ectopically in lineage branches that normally do not express this gene. Such expression is dependent on the Hox gene mab-5, as it is in branches where egl-5 is normally expressed. Ectopic egl-5 expression is also dependent on the Wnt pathway. Thus, sop-3 contributes to the combinatorial control of egl-5 by blocking egl-5 activation by MAB-5 and the Wnt pathway in inappropriate lineage branches.",
keywords = "C. elegans, Homeobox gene, Regulation, Sop-3, Transcription, Wnt signaling",
author = "H. Zhang and Emmons, {Scott W.}",
year = "2001",
language = "English (US)",
volume = "128",
pages = "767--777",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "5",

}

TY - JOUR

T1 - The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox gene expression

AU - Zhang, H.

AU - Emmons, Scott W.

PY - 2001

Y1 - 2001

N2 - We describe the properties of a new gene, sop-3, that is required for the regulated expression of a C. elegans Hox gene, egl-5, in a postembryonic neuroectodermal cell lineage. Regulated expression of egl-5 in this cell lineage is necessary for development of the sensory rays of the male tail. sop-3 encodes a predicted novel protein of 1475 amino acids without clear homologs in other organisms. However, the sequence contains motifs consisting of homopolymeric runs of amino acids found in several other transcriptional regulators, some of which also act in Hox gene regulatory pathways. The genetic properties of sop-3 are very similar to those of sop-1, which encodes a component of the transcriptional Mediator complex, and mutations in the two genes are synthetic lethal. This suggests that SOP-3 may act at the level of the Mediator complex in regulating transcription initiation. In a sop-3 loss-of-function background, egl-5 is expressed ectopically in lineage branches that normally do not express this gene. Such expression is dependent on the Hox gene mab-5, as it is in branches where egl-5 is normally expressed. Ectopic egl-5 expression is also dependent on the Wnt pathway. Thus, sop-3 contributes to the combinatorial control of egl-5 by blocking egl-5 activation by MAB-5 and the Wnt pathway in inappropriate lineage branches.

AB - We describe the properties of a new gene, sop-3, that is required for the regulated expression of a C. elegans Hox gene, egl-5, in a postembryonic neuroectodermal cell lineage. Regulated expression of egl-5 in this cell lineage is necessary for development of the sensory rays of the male tail. sop-3 encodes a predicted novel protein of 1475 amino acids without clear homologs in other organisms. However, the sequence contains motifs consisting of homopolymeric runs of amino acids found in several other transcriptional regulators, some of which also act in Hox gene regulatory pathways. The genetic properties of sop-3 are very similar to those of sop-1, which encodes a component of the transcriptional Mediator complex, and mutations in the two genes are synthetic lethal. This suggests that SOP-3 may act at the level of the Mediator complex in regulating transcription initiation. In a sop-3 loss-of-function background, egl-5 is expressed ectopically in lineage branches that normally do not express this gene. Such expression is dependent on the Hox gene mab-5, as it is in branches where egl-5 is normally expressed. Ectopic egl-5 expression is also dependent on the Wnt pathway. Thus, sop-3 contributes to the combinatorial control of egl-5 by blocking egl-5 activation by MAB-5 and the Wnt pathway in inappropriate lineage branches.

KW - C. elegans

KW - Homeobox gene

KW - Regulation

KW - Sop-3

KW - Transcription

KW - Wnt signaling

UR - http://www.scopus.com/inward/record.url?scp=0035092460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035092460&partnerID=8YFLogxK

M3 - Article

C2 - 11171401

AN - SCOPUS:0035092460

VL - 128

SP - 767

EP - 777

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 5

ER -