The New York Consortium on Membrane Protein Structure (NYCOMPS): A high-throughput platform for structural genomics of integral membrane proteins

James Love; Filippo Mancia; Lawrence Shapiro; Marco Punta; Burkhard Rost; Mark Girvin; Da Neng Wang; Ming Zhou; John F. Hunt; Thomas Szyperski; Eric Gouaux; Roderick MacKinnon; Ann McDermott; Barry Honig; Masayori Inouye; Gaetano Montelione; Wayne A. Hendrickson

doi:10.1007/s10969-010-9094-7

The New York Consortium on Membrane Protein Structure (NYCOMPS): A high-throughput platform for structural genomics of integral membrane proteins

James Love, Filippo Mancia, Lawrence Shapiro, Marco Punta, Burkhard Rost, Mark Girvin, Da Neng Wang, Ming Zhou, John F. Hunt, Thomas Szyperski, Eric Gouaux, Roderick MacKinnon, Ann McDermott, Barry Honig, Masayori Inouye, Gaetano Montelione, Wayne A. Hendrickson

Biochemistry

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.

Original language	English (US)
Pages (from-to)	191-199
Number of pages	9
Journal	Journal of Structural and Functional Genomics
Volume	11
Issue number	3
DOIs	https://doi.org/10.1007/s10969-010-9094-7
State	Published - Sep 2010

Keywords

High throughput
Membrane proteins
NMR
Structural genomics
X-ray

ASJC Scopus subject areas

Structural Biology
Biochemistry
Genetics

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10.1007/s10969-010-9094-7

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Love, J., Mancia, F., Shapiro, L., Punta, M., Rost, B., Girvin, M., Wang, D. N., Zhou, M., Hunt, J. F., Szyperski, T., Gouaux, E., MacKinnon, R., McDermott, A., Honig, B., Inouye, M., Montelione, G., & Hendrickson, W. A. (2010). The New York Consortium on Membrane Protein Structure (NYCOMPS): A high-throughput platform for structural genomics of integral membrane proteins. Journal of Structural and Functional Genomics, 11(3), 191-199. https://doi.org/10.1007/s10969-010-9094-7

Love, J, Mancia, F, Shapiro, L, Punta, M, Rost, B, Girvin, M, Wang, DN, Zhou, M, Hunt, JF, Szyperski, T, Gouaux, E, MacKinnon, R, McDermott, A, Honig, B, Inouye, M, Montelione, G & Hendrickson, WA 2010, 'The New York Consortium on Membrane Protein Structure (NYCOMPS): A high-throughput platform for structural genomics of integral membrane proteins', Journal of Structural and Functional Genomics, vol. 11, no. 3, pp. 191-199. https://doi.org/10.1007/s10969-010-9094-7

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abstract = "The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.",

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author = "James Love and Filippo Mancia and Lawrence Shapiro and Marco Punta and Burkhard Rost and Mark Girvin and Wang, {Da Neng} and Ming Zhou and Hunt, {John F.} and Thomas Szyperski and Eric Gouaux and Roderick MacKinnon and Ann McDermott and Barry Honig and Masayori Inouye and Gaetano Montelione and Hendrickson, {Wayne A.}",

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The New York Consortium on Membrane Protein Structure (NYCOMPS): A high-throughput platform for structural genomics of integral membrane proteins

Abstract

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