The New York Consortium on Membrane Protein Structure (NYCOMPS): A high-throughput platform for structural genomics of integral membrane proteins

James Love, Filippo Mancia, Lawrence Shapiro, Marco Punta, Burkhard Rost, Mark Girvin, Da Neng Wang, Ming Zhou, John F. Hunt, Thomas Szyperski, Eric Gouaux, Roderick MacKinnon, Ann McDermott, Barry Honig, Masayori Inouye, Gaetano Montelione, Wayne A. Hendrickson

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.

Original languageEnglish (US)
Pages (from-to)191-199
Number of pages9
JournalJournal of Structural and Functional Genomics
Volume11
Issue number3
DOIs
StatePublished - Sep 2010

Keywords

  • High throughput
  • Membrane proteins
  • NMR
  • Structural genomics
  • X-ray

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

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    Love, J., Mancia, F., Shapiro, L., Punta, M., Rost, B., Girvin, M., Wang, D. N., Zhou, M., Hunt, J. F., Szyperski, T., Gouaux, E., MacKinnon, R., McDermott, A., Honig, B., Inouye, M., Montelione, G., & Hendrickson, W. A. (2010). The New York Consortium on Membrane Protein Structure (NYCOMPS): A high-throughput platform for structural genomics of integral membrane proteins. Journal of Structural and Functional Genomics, 11(3), 191-199. https://doi.org/10.1007/s10969-010-9094-7