The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses

Xabier Agirrezabala, Eduardo Méndez-López, Gorka Lasso, M. Amelia Sánchez-Pina, Miguel Aranda, Mikel Valle

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Flexible filamentous viruses include economically important plant pathogens. Their viral particles contain several hundred copies of a helically arrayed coat protein (CP) protecting a (+)ssRNA. We describe here a structure at 3.9 Å resolution, from electron cryomicroscopy, of Pepino mosaic virus (PepMV), a representative of the genus Potexvirus (family Alphaflexiviridae). Our results allow modeling of the CP and its interactions with viral RNA. The overall fold of PepMV CP resembles that of nucleoproteins (NPs) from the genus Phlebovirus (family Bunyaviridae), a group of enveloped (-)ssRNA viruses. The main difference between potexvirus CP and phlebovirus NP is in their C-terminal extensions, which appear to determine the characteristics of the distinct multimeric assemblies - a flexuous, helical rod or a loose ribonucleoprotein. The homology suggests gene transfer between eukaryotic (+) and (-)ssRNA viruses.

Original languageEnglish (US)
Article numbere11795
JournaleLife
Volume4
Issue numberDECEMBER2015
DOIs
StatePublished - Dec 16 2015
Externally publishedYes

Fingerprint

Plant Viruses
Nucleoproteins
Capsid Proteins
Viruses
Animals
Phlebovirus
Potexvirus
Mosaic Viruses
Bunyaviridae
Cryoelectron Microscopy
Ribonucleoproteins
Viral RNA
Gene transfer
Virion
Pathogens
Electrons
Genes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses. / Agirrezabala, Xabier; Méndez-López, Eduardo; Lasso, Gorka; Sánchez-Pina, M. Amelia; Aranda, Miguel; Valle, Mikel.

In: eLife, Vol. 4, No. DECEMBER2015, e11795, 16.12.2015.

Research output: Contribution to journalArticle

Agirrezabala, Xabier ; Méndez-López, Eduardo ; Lasso, Gorka ; Sánchez-Pina, M. Amelia ; Aranda, Miguel ; Valle, Mikel. / The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses. In: eLife. 2015 ; Vol. 4, No. DECEMBER2015.
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