The Mycobacterium xenopi GyrA protein splicing element: Characterization of a minimal intein

Amalio Telenti, Maurice Southworth, Fernando Alcaide, Sabine Daugelat, William R. Jacobs, Francine B. Perler

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

The 198-amino-acid in-frame insertion in the gyrA gene of Mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). Comparison with other mycobacterial gyrA inteins suggests that the M. xenopi intein underwent a complex series of events including (i) removal of 222 amino acids that encompass most of the central intein domain, and (ii) addition of a linker of unrelated residues. This naturally occurring genetic rearrangement is a representative characteristics of the taxon. The deletion process removes the conserved motifs involved in homing endonuclease activity. The linker insertion represents a structural requirement, as its mutation resulted in failure to splice. The M. xenopi GyrA intein thus provides a paradigm for a minimal protein splicing element.

Original languageEnglish (US)
Pages (from-to)6378-6382
Number of pages5
JournalJournal of Bacteriology
Volume179
Issue number20
DOIs
StatePublished - Oct 1997

Fingerprint

Mycobacterium xenopi
Inteins
Protein Splicing
DNA Gyrase
Amino Acids
Endonucleases
Mutation
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

The Mycobacterium xenopi GyrA protein splicing element : Characterization of a minimal intein. / Telenti, Amalio; Southworth, Maurice; Alcaide, Fernando; Daugelat, Sabine; Jacobs, William R.; Perler, Francine B.

In: Journal of Bacteriology, Vol. 179, No. 20, 10.1997, p. 6378-6382.

Research output: Contribution to journalArticle

Telenti, Amalio ; Southworth, Maurice ; Alcaide, Fernando ; Daugelat, Sabine ; Jacobs, William R. ; Perler, Francine B. / The Mycobacterium xenopi GyrA protein splicing element : Characterization of a minimal intein. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 20. pp. 6378-6382.
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