The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon induces Th-1 type response deleterious to protection in mice

Rashmi Pasricha, Neeraj K. Saini, Nisha Rathor, Rakesh Pathak, Rajesh Sinha, Mandira Varma-Basil, Kiran Mishra, Vani Brahmachari, Mridula Bose

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lipoproteins are known to be effective immunogens and affect both innate and adaptive immunity. The lprN gene of Mycobacterium tuberculosis has been predicted to encode for a putative lipoprotein in silico. Here, we studied its function as an immunogen by in vivo studies in mice. The recombinant LprN protein, expressed and purified in Escherichia coli, triggered a cell-mediated immune response in BALB/c mice. This was observed by significantly higher T-cell proliferation and increased production of TNF-α and IFN-γ cytokines. However, pre-exposure to LprN protein failed to provide protection in mice after challenge with a virulent strain of M. tuberculosis. Histological examination showed an increase in tissue destruction in experimental animals, indicating an immunogenic potential for LprN protein that enhanced the virulence of bacilli. The LprN protein of Mycobacterium tuberculosis may have a pathogenic potential to play important role in long term disease maintenance in host.

Original languageEnglish (US)
Pages (from-to)188-196
Number of pages9
JournalPathogens and Disease
Volume72
Issue number3
DOIs
StatePublished - Dec 1 2014
Externally publishedYes

Fingerprint

Operon
Mycobacterium tuberculosis
Recombinant Proteins
Lipoproteins
Proteins
Adaptive Immunity
Innate Immunity
Computer Simulation
Bacillus
Virulence
Maintenance
Cell Proliferation
Cytokines
Escherichia coli
T-Lymphocytes
Genes

Keywords

  • Immunogen
  • LprN
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon induces Th-1 type response deleterious to protection in mice. / Pasricha, Rashmi; Saini, Neeraj K.; Rathor, Nisha; Pathak, Rakesh; Sinha, Rajesh; Varma-Basil, Mandira; Mishra, Kiran; Brahmachari, Vani; Bose, Mridula.

In: Pathogens and Disease, Vol. 72, No. 3, 01.12.2014, p. 188-196.

Research output: Contribution to journalArticle

Pasricha, R, Saini, NK, Rathor, N, Pathak, R, Sinha, R, Varma-Basil, M, Mishra, K, Brahmachari, V & Bose, M 2014, 'The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon induces Th-1 type response deleterious to protection in mice', Pathogens and Disease, vol. 72, no. 3, pp. 188-196. https://doi.org/10.1111/2049-632X.12200
Pasricha, Rashmi ; Saini, Neeraj K. ; Rathor, Nisha ; Pathak, Rakesh ; Sinha, Rajesh ; Varma-Basil, Mandira ; Mishra, Kiran ; Brahmachari, Vani ; Bose, Mridula. / The Mycobacterium tuberculosis recombinant LprN protein of mce4 operon induces Th-1 type response deleterious to protection in mice. In: Pathogens and Disease. 2014 ; Vol. 72, No. 3. pp. 188-196.
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