Abstract
Phenolic glycolipids (PGLs) are non-covalently bound components of the outer membrane of many clinically relevant mycobacterial pathogens, and play important roles in pathogen biology. We report a mutational analysis that conclusively demonstrates that the conserved acyltransferase-encoding gene papA5 is essential for PGL production. In addition, we provide an in vitro acyltransferase activity analysis that establishes proof of principle for the competency of PapA5 to utilize diol-containing polyketide compounds of mycobacterial origin as acyl-acceptor substrates. Overall, the results reported herein are in line with a model in which PapA5 catalyses the acylation of diol-containing polyketides to form PGLs. These studies advance our understanding of the biosynthesis of an important group of mycobacterial glycolipids and suggest that PapA5 might be an attractive target for exploring the development of antivirulence drugs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1379-1387 |
| Number of pages | 9 |
| Journal | Microbiology |
| Volume | 158 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2012 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
Cite this
The mycobacterial acyltransferase PapA5 is required for biosynthesis of cell wall-associated phenolic glycolipids. / Chavadi, Sivagami Sundaram; Onwueme, Kenolisa C.; Edupuganti, Uthamaphani R.; Jerome, Jeff; Chatterjee, Delphi; Soll, Clifford E.; Quadri, Luis E N.
In: Microbiology, Vol. 158, No. 5, 05.2012, p. 1379-1387.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The mycobacterial acyltransferase PapA5 is required for biosynthesis of cell wall-associated phenolic glycolipids
AU - Chavadi, Sivagami Sundaram
AU - Onwueme, Kenolisa C.
AU - Edupuganti, Uthamaphani R.
AU - Jerome, Jeff
AU - Chatterjee, Delphi
AU - Soll, Clifford E.
AU - Quadri, Luis E N
PY - 2012/5
Y1 - 2012/5
N2 - Phenolic glycolipids (PGLs) are non-covalently bound components of the outer membrane of many clinically relevant mycobacterial pathogens, and play important roles in pathogen biology. We report a mutational analysis that conclusively demonstrates that the conserved acyltransferase-encoding gene papA5 is essential for PGL production. In addition, we provide an in vitro acyltransferase activity analysis that establishes proof of principle for the competency of PapA5 to utilize diol-containing polyketide compounds of mycobacterial origin as acyl-acceptor substrates. Overall, the results reported herein are in line with a model in which PapA5 catalyses the acylation of diol-containing polyketides to form PGLs. These studies advance our understanding of the biosynthesis of an important group of mycobacterial glycolipids and suggest that PapA5 might be an attractive target for exploring the development of antivirulence drugs.
AB - Phenolic glycolipids (PGLs) are non-covalently bound components of the outer membrane of many clinically relevant mycobacterial pathogens, and play important roles in pathogen biology. We report a mutational analysis that conclusively demonstrates that the conserved acyltransferase-encoding gene papA5 is essential for PGL production. In addition, we provide an in vitro acyltransferase activity analysis that establishes proof of principle for the competency of PapA5 to utilize diol-containing polyketide compounds of mycobacterial origin as acyl-acceptor substrates. Overall, the results reported herein are in line with a model in which PapA5 catalyses the acylation of diol-containing polyketides to form PGLs. These studies advance our understanding of the biosynthesis of an important group of mycobacterial glycolipids and suggest that PapA5 might be an attractive target for exploring the development of antivirulence drugs.
UR - http://www.scopus.com/inward/record.url?scp=84860528767&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860528767&partnerID=8YFLogxK
U2 - 10.1099/mic.0.057869-0
DO - 10.1099/mic.0.057869-0
M3 - Article
C2 - 22361940
AN - SCOPUS:84860528767
VL - 158
SP - 1379
EP - 1387
JO - Microbiology (United Kingdom)
JF - Microbiology (United Kingdom)
SN - 1350-0872
IS - 5
ER -