@article{9fca752a9869412e859bb0254f8d0f86,
title = "The Msx1 homeoprotein recruits polycomb to the nuclear periphery during development",
abstract = "Control of gene expression during development requires the concerted action of sequence-specific transcriptional regulators and epigenetic modifiers, which are spatially coordinated within the nucleus through mechanisms that are poorly understood. Here we show that transcriptional repression by the Msx1 homeoprotein in myoblast cells requires the recruitment of Polycomb to target genes located at the nuclear periphery. Target genes repressed by Msx1 display an Msx1-dependent enrichment of Polycomb-directed trimethylation of lysine 27 on histone H3 (H3K27me3). Association of Msx1 with the Polycomb complex is required for repression and regulation of myoblast differentiation. Furthermore, Msx1 promotes a dynamic spatial redistribution of the H3K27me3 repressive mark to the nuclear periphery in myoblast cells and the developing limb in vivo. Our findings illustrate a hitherto unappreciated spatial coordination of transcription factors with the Polycomb complex for appropriate regulation of gene expression programs during development.",
author = "Jingqiang Wang and Kumar, {Roshan M.} and Biggs, {Vanessa J.} and Hansol Lee and Yun Chen and Kagey, {Michael H.} and Young, {Richard A.} and Cory Abate-Shen",
note = "Funding Information: We are indebted to Drs. Danny Reinberg and Raphael Margueron for providing reagents and many helpful discussions, Dr. Murty Vundavalli for assistance with FISH, and Dr. Manus Biggs for assistance with ImageJ. We are grateful to Dr. Robert Maxson for providing the Msx1 and Msx2 conditional mice, Drs. David Sassoon and Virginia E. Papaioannou for plasmids, and Susan Morton and Dr. Thomas M. Jessell for the Msx antibody. We thank Lejuan F. Chatman, Celia D. Keim, and Songyan Han for technical assistance, Garrett Frampton, David Orlando, and Stuart Levine for computational support with ChIP-Seq analyses, George Bell and the Whitehead Bioinformatics and Research Computing group for assistance with expression analyses, members of the Young and Abate-Shen laboratories for helpful discussions, and Dr. Michael Shen for helpful discussions and comments on the manuscript. R.M.K. was supported by an American Cancer Society postdoctoral fellowship. This work was supported by The T.J. Martell Foundation for Leukemia, Cancer and AIDS Research (C.A.-S.) and by grants HG002668 (R.A.Y.) and HD029446 (C.A.-S.). ",
year = "2011",
month = sep,
day = "13",
doi = "10.1016/j.devcel.2011.07.003",
language = "English (US)",
volume = "21",
pages = "575--588",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "3",
}