The mouse chorionic gonadotropin β-subunit-like (muCGβ1) molecule produced by tumor cells elicits the switch of T-cell immunity response from TH2 to TH1 in mice immunized with DNA vaccine based on rhesus monkey homologous CGβ (rmCGβ)

Shu Qun Shi, Jing Pian Peng, Li Xu, Ying Yang, Yun Chen

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background. CGβ is expressed not only in placenta, but also in a wide range of tumors. To study DNA vaccine based on xenogeneic CGβ for cancer immuno-therapy, we investigated whether rhesus monkey CGβ (rmCGβ) DNA vaccine could induce protective T-cell responses and humoral responses in mouse. Methods. We constructed a plasmid containing the rmCGβ coding sequence. Two cloned syngeneic SP2/0 myeloma cell lines that stably express muCGβ1 (SP2/0-muCGβ1) and HN (SP2/0-HN) protein were established. Inoculation of these cell lines was made into mice that had been immunized with DNA vaccine. Specific IgG and IgG type were measured by ELISA and the cytokine expression was detected with RT-PCR. To measure the lymphocyte metabolic activity, the MTS assay was used. Results. After injection of SP2/0-muCGβ into mice that had been immunized with DNA vaccine, a significant increase in the IgG2a specific to the antigen (p < 0.05) and a decrease in the specific IgG1 (p < 0.05) were measured. The expression of TH1 but not TH2 cytokines, including IFN-γ and IL-2, were detected in the splenocytes. However, injection of tumor cells expressing irrelevant or mock molecules into immunized mice could not induce these changes. The survival rate of vaccine-immunized mice injected with SP2/0-muCGβ1 was as high as 58.3% after 55 days. Conclusions. The rmCGβ DNA vaccine has proved to be a potential strategy for protection against tumors with homologous molecules. The muCGβ1 produced by tumors is able to elicit an immunity switch from TH2 to TH1 in vaccinated mice.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalJournal of Gene Medicine
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 1 2005
Externally publishedYes

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Keywords

  • CGβ
  • Immuno-protection
  • Switch
  • T-cell immunity
  • Tumor vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Drug Discovery
  • Genetics(clinical)

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