The monoclonal antibody against the major diagnostic antigen of Paracoccidioides brasiliensis mediates immune protection in infected BALB/c mice challenged intratracheally with the fungus

R. Buissa-Filho, R. Puccia, A. F. Marques, F. A. Pinto, J. E. Muñoz, Joshua D. Nosanchuk, L. R. Travassos, C. P. Taborda

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Abstract

The protective role of specific antibodies against Paracoccidioides brasiliensis is controversial. In the present study, we analyzed the effects of monoclonal antibodies on the major diagnostic antigen (gp43) using in vitro and in vivo P. brasiliensis infection models. The passive administration of some monoclonal antibodies (MAbs) before and after intratracheal or intravenous infections led to a reduced fungal burden and decreased pulmonary inflammation. The protection mediated by MAb 3E, the most efficient MAb in the reduction of fungal burden, was associated with the enhanced phagocytosis of P. brasiliensis yeast cells by J774.16, MH-S, or primary macrophages. The ingestion of opsonized yeast cells led to an increase in NO production by macrophages. Passive immunization with MAb 3E induced enhanced levels of gamma interferon in the lungs of infected mice. The reactivity of MAb 3E against a panel of gp43-derived peptides suggested that the sequence NHVRIPIGWAV contains the binding epitope. The present work shows that some but not all MAbs against gp43 can reduce the fungal burden and identifies a new peptide candidate for vaccine development.

Original languageEnglish (US)
Pages (from-to)3321-3328
Number of pages8
JournalInfection and Immunity
Volume76
Issue number7
DOIs
StatePublished - Jul 2008

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Paracoccidioides
Fungi
Monoclonal Antibodies
Antigens
Yeasts
Macrophages
Subunit Vaccines
Passive Immunization
Infection
Phagocytosis
Interferon-gamma
Epitopes
Pneumonia
Eating
Lung
Peptides
Antibodies

ASJC Scopus subject areas

  • Immunology

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The monoclonal antibody against the major diagnostic antigen of Paracoccidioides brasiliensis mediates immune protection in infected BALB/c mice challenged intratracheally with the fungus. / Buissa-Filho, R.; Puccia, R.; Marques, A. F.; Pinto, F. A.; Muñoz, J. E.; Nosanchuk, Joshua D.; Travassos, L. R.; Taborda, C. P.

In: Infection and Immunity, Vol. 76, No. 7, 07.2008, p. 3321-3328.

Research output: Contribution to journalArticle

Buissa-Filho, R. ; Puccia, R. ; Marques, A. F. ; Pinto, F. A. ; Muñoz, J. E. ; Nosanchuk, Joshua D. ; Travassos, L. R. ; Taborda, C. P. / The monoclonal antibody against the major diagnostic antigen of Paracoccidioides brasiliensis mediates immune protection in infected BALB/c mice challenged intratracheally with the fungus. In: Infection and Immunity. 2008 ; Vol. 76, No. 7. pp. 3321-3328.
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