The metabolic consequences of altered glucose transporter expression in transgenic mice

E. B. Katz, R. Burcelin, T. S. Tsao, A. E. Stenbit, Maureen J. Charron

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Glucose transporters are a family of membrane proteins which mediate glucose uptake across the cell membrane. The facilitative glucose transporter proteins are products of unique genes and are expressed in a tissue-specific manner. They are very similar structurally, containing 12 putative membrane spanning domains. Functionally they vary in their affinity for glucose and sensitivity to hormones such as insulin. Glucose homeostasis depends mainly on controlled changes in glucose transport in insulin-responsive tissues such as skeletal muscle and adipose cells where both glucose transporter 1 and glucose transporter 4 are expressed. Glucose transporter 4 is the major glucose transporter in these tissues and translocates from an intracellular vesicle to the cell membrane in response to insulin. Alterations of the level of expression of these glucose transporters should result in changes in insulin sensitivity and modification of whole-body metabolism To test these hypotheses transgenic mouse models have been generated which overexpress glucose transporters in specific tissues or in the whole body. Glucose transporter 1 and glucose transporter 4 have been overexpressed specifically in skeletal muscle and glucose transporter 4 specifically in adipose tissue. Mice have also been made which overexpress glucose transporter 4 in the whole body. Using homologous recombination technology to disrupt the glucose transporter 4 gene, a 'knockout' mouse has been created which expresses no glucose transporter 4. The metabolic consequences of these genetic manipulations on the level of expression of glucose transporters in the mouse are reviewed. The future applications of transgenic mouse technology in creating models which mimic human diseases are also discussed.

Original languageEnglish (US)
Pages (from-to)639-652
Number of pages14
JournalJournal of Molecular Medicine
Volume74
Issue number11
DOIs
StatePublished - 1996

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Facilitative Glucose Transport Proteins
Transgenic Mice
Glucose
Insulin
Skeletal Muscle
Cell Membrane
Technology
Gene Knockout Techniques
Homologous Recombination
Knockout Mice
Muscle Cells

Keywords

  • Glucose homeostasis
  • Glucose transporter 4
  • Insulin action
  • Transgenic mice

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The metabolic consequences of altered glucose transporter expression in transgenic mice. / Katz, E. B.; Burcelin, R.; Tsao, T. S.; Stenbit, A. E.; Charron, Maureen J.

In: Journal of Molecular Medicine, Vol. 74, No. 11, 1996, p. 639-652.

Research output: Contribution to journalArticle

Katz, E. B. ; Burcelin, R. ; Tsao, T. S. ; Stenbit, A. E. ; Charron, Maureen J. / The metabolic consequences of altered glucose transporter expression in transgenic mice. In: Journal of Molecular Medicine. 1996 ; Vol. 74, No. 11. pp. 639-652.
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