The major rheumatoid factor Cross-reactive idiotype in rheumatic disease

Vincent R. Bonagura, Benvenuto Pernis, Nicodemo Agostino, Norman Todd Ilowite, Lynda Hatam, Josiah F. Wedgwood

Research output: Contribution to journalArticle

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Abstract

The major rheumatoid factor cross-reactive idiotype (RCRI), a tertiary structure formed by both light and heavy chains, is found on 60% of all monoclonal IgM kappa RFs. To determine if the RCRI is expressed in patients with rheumatic disease, we used polyclonal rabbit anti-idiotypic antibodies to detect RCRI in sera and in pokeweed mitogen cultures of blood mononuclear cells (PBM) from patients with rheumatoid arthritis (RA) and juvenile rheumatoid arthritis (JRA). We detected increased expression of RCRI + plasma cells in PWM cultures, and in sera from these patients. We have determined that some 7S IgM molecules from RF+ RA patients are RCRI + and can bind IgG in a sensitive RF ELISA. We have also observed that the CD5+ B cell subset, which is responsible for autoantibody production, generates RCRI+ antibodies. We review these data and discuss the relationship of the idiotypic network of interacting antibodies with rheumatic disease.

Original languageEnglish (US)
Pages (from-to)139-151
Number of pages13
JournalInternational Reviews of Immunology
Volume5
Issue number2
DOIs
StatePublished - 1989

Fingerprint

Rheumatoid Factor
Rheumatic Diseases
Immunoglobulin M
Rheumatoid Arthritis
B-Lymphocyte Subsets
Pokeweed Mitogens
Juvenile Arthritis
Antibodies
Plasma Cells
Serum
Autoantibodies
Anti-Idiotypic Antibodies
Blood Cells
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Rabbits
Light

Keywords

  • Cross-reactive idiolype
  • Rheumatic disease
  • Rheumatoid factor

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

The major rheumatoid factor Cross-reactive idiotype in rheumatic disease. / Bonagura, Vincent R.; Pernis, Benvenuto; Agostino, Nicodemo; Ilowite, Norman Todd; Hatam, Lynda; Wedgwood, Josiah F.

In: International Reviews of Immunology, Vol. 5, No. 2, 1989, p. 139-151.

Research output: Contribution to journalArticle

Bonagura, Vincent R. ; Pernis, Benvenuto ; Agostino, Nicodemo ; Ilowite, Norman Todd ; Hatam, Lynda ; Wedgwood, Josiah F. / The major rheumatoid factor Cross-reactive idiotype in rheumatic disease. In: International Reviews of Immunology. 1989 ; Vol. 5, No. 2. pp. 139-151.
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