The lysine 65 residue in HIV-1 reverse transcriptase function and in nucleoside analog drug resistance

Scott J. Garforth; Chisanga Lwatula; Vinayaka R. Prasad

doi:10.3390/v6104080

The lysine 65 residue in HIV-1 reverse transcriptase function and in nucleoside analog drug resistance

Scott J. Garforth, Chisanga Lwatula, Vinayaka R. Prasad

Research output: Contribution to journal › Review article › peer-review

17 Scopus citations

Abstract

Mutations in HIV-1 reverse transcriptase (RT) that confer nucleoside analog RT inhibitor resistance have highlighted the functional importance of several active site residues (M184, Q151 and K65) in RT catalytic function. Of these, K65 residue is notable due to its pivotal position in the dNTP-binding pocket, its involvement in nucleoside analog resistance and polymerase fidelity. This review focuses on K65 residue and summarizes a substantial body of biochemical and structural studies of its role in RT function and the functional consequences of the K65R mutation.

Original language	English (US)
Pages (from-to)	4080-4094
Number of pages	15
Journal	Viruses
Volume	6
Issue number	10
DOIs	https://doi.org/10.3390/v6104080
State	Published - Oct 23 2014

Keywords

HIV-1 drug resistance
HIV-1 reverse transcriptase
K65R mutation
NRTI analog resistance
Polymerase fidelity
Reverse transcriptase function

ASJC Scopus subject areas

Infectious Diseases
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v6104080

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title = "The lysine 65 residue in HIV-1 reverse transcriptase function and in nucleoside analog drug resistance",

abstract = "Mutations in HIV-1 reverse transcriptase (RT) that confer nucleoside analog RT inhibitor resistance have highlighted the functional importance of several active site residues (M184, Q151 and K65) in RT catalytic function. Of these, K65 residue is notable due to its pivotal position in the dNTP-binding pocket, its involvement in nucleoside analog resistance and polymerase fidelity. This review focuses on K65 residue and summarizes a substantial body of biochemical and structural studies of its role in RT function and the functional consequences of the K65R mutation.",

keywords = "HIV-1 drug resistance, HIV-1 reverse transcriptase, K65R mutation, NRTI analog resistance, Polymerase fidelity, Reverse transcriptase function",

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AU - Garforth, Scott J.

AU - Lwatula, Chisanga

AU - Prasad, Vinayaka R.

PY - 2014/10/23

Y1 - 2014/10/23

N2 - Mutations in HIV-1 reverse transcriptase (RT) that confer nucleoside analog RT inhibitor resistance have highlighted the functional importance of several active site residues (M184, Q151 and K65) in RT catalytic function. Of these, K65 residue is notable due to its pivotal position in the dNTP-binding pocket, its involvement in nucleoside analog resistance and polymerase fidelity. This review focuses on K65 residue and summarizes a substantial body of biochemical and structural studies of its role in RT function and the functional consequences of the K65R mutation.

AB - Mutations in HIV-1 reverse transcriptase (RT) that confer nucleoside analog RT inhibitor resistance have highlighted the functional importance of several active site residues (M184, Q151 and K65) in RT catalytic function. Of these, K65 residue is notable due to its pivotal position in the dNTP-binding pocket, its involvement in nucleoside analog resistance and polymerase fidelity. This review focuses on K65 residue and summarizes a substantial body of biochemical and structural studies of its role in RT function and the functional consequences of the K65R mutation.

KW - HIV-1 drug resistance

KW - HIV-1 reverse transcriptase

KW - K65R mutation

KW - NRTI analog resistance

KW - Polymerase fidelity

KW - Reverse transcriptase function

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The lysine 65 residue in HIV-1 reverse transcriptase function and in nucleoside analog drug resistance

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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