The life of an mRNA in space and time

Ya'Ara Ben-Ari, Yehuda Brody, Noa Kinor, Amir Mor, Toshiro Tsukamoto, David L. Spector, Robert H. Singer, Yaron Shav-Tal

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Nuclear transcribed genes produce mRNA transcripts destined to travel from the site of transcription to the cytoplasm for protein translation. Certain transcripts can be further localized to specific cytoplasmic regions. We examined the life cycle of a transcribed β-actin mRNA throughout gene expression and localization, in a cell system that allows the in vivo detection of the gene locus, the transcribed mRNAs and the cytoplasmic β-actin protein that integrates into the actin cytoskeleton. Quantification showed that RNA polymerase II elongation progressed at a rate of 3.3 kb/minute and that transactivator binding to the promoter was transient (40 seconds), and demonstrated the unique spatial structure of the coding and non-coding regions of the integrated gene within the transcription site. The rates of gene induction were measured during interphase and after mitosis, demonstrating that daughter cells were not synchronized in respect to transcription initiation of the studied gene. Comparison of the spatial and temporal kinetics of nucleoplasmic and cytoplasmic mRNA transport showed that the β-actin-localization response initiates from the existing cytoplasmic mRNA pool and not from the newly synthesized transcripts arising after gene induction. It was also demonstrated that mechanisms of random movement were predominant in mediating the efficient translocation of mRNA in the eukaryotic cell.

Original languageEnglish (US)
Pages (from-to)1761-1774
Number of pages14
JournalJournal of Cell Science
Volume123
Issue number10
DOIs
StatePublished - May 15 2010

Fingerprint

Messenger RNA
Genes
Actins
Trans-Activators
RNA Polymerase II
Interphase
Protein Biosynthesis
Eukaryotic Cells
Life Cycle Stages
Actin Cytoskeleton
Mitosis
Cytoplasm
Gene Expression
Proteins

Keywords

  • Gene expression
  • Live cell imaging
  • mRNA dynamics
  • Transcription

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Ben-Ari, YA., Brody, Y., Kinor, N., Mor, A., Tsukamoto, T., Spector, D. L., ... Shav-Tal, Y. (2010). The life of an mRNA in space and time. Journal of Cell Science, 123(10), 1761-1774. https://doi.org/10.1242/jcs.062638

The life of an mRNA in space and time. / Ben-Ari, Ya'Ara; Brody, Yehuda; Kinor, Noa; Mor, Amir; Tsukamoto, Toshiro; Spector, David L.; Singer, Robert H.; Shav-Tal, Yaron.

In: Journal of Cell Science, Vol. 123, No. 10, 15.05.2010, p. 1761-1774.

Research output: Contribution to journalArticle

Ben-Ari, YA, Brody, Y, Kinor, N, Mor, A, Tsukamoto, T, Spector, DL, Singer, RH & Shav-Tal, Y 2010, 'The life of an mRNA in space and time', Journal of Cell Science, vol. 123, no. 10, pp. 1761-1774. https://doi.org/10.1242/jcs.062638
Ben-Ari YA, Brody Y, Kinor N, Mor A, Tsukamoto T, Spector DL et al. The life of an mRNA in space and time. Journal of Cell Science. 2010 May 15;123(10):1761-1774. https://doi.org/10.1242/jcs.062638
Ben-Ari, Ya'Ara ; Brody, Yehuda ; Kinor, Noa ; Mor, Amir ; Tsukamoto, Toshiro ; Spector, David L. ; Singer, Robert H. ; Shav-Tal, Yaron. / The life of an mRNA in space and time. In: Journal of Cell Science. 2010 ; Vol. 123, No. 10. pp. 1761-1774.
@article{d4766378b8e9423793e490b056458015,
title = "The life of an mRNA in space and time",
abstract = "Nuclear transcribed genes produce mRNA transcripts destined to travel from the site of transcription to the cytoplasm for protein translation. Certain transcripts can be further localized to specific cytoplasmic regions. We examined the life cycle of a transcribed β-actin mRNA throughout gene expression and localization, in a cell system that allows the in vivo detection of the gene locus, the transcribed mRNAs and the cytoplasmic β-actin protein that integrates into the actin cytoskeleton. Quantification showed that RNA polymerase II elongation progressed at a rate of 3.3 kb/minute and that transactivator binding to the promoter was transient (40 seconds), and demonstrated the unique spatial structure of the coding and non-coding regions of the integrated gene within the transcription site. The rates of gene induction were measured during interphase and after mitosis, demonstrating that daughter cells were not synchronized in respect to transcription initiation of the studied gene. Comparison of the spatial and temporal kinetics of nucleoplasmic and cytoplasmic mRNA transport showed that the β-actin-localization response initiates from the existing cytoplasmic mRNA pool and not from the newly synthesized transcripts arising after gene induction. It was also demonstrated that mechanisms of random movement were predominant in mediating the efficient translocation of mRNA in the eukaryotic cell.",
keywords = "Gene expression, Live cell imaging, mRNA dynamics, Transcription",
author = "Ya'Ara Ben-Ari and Yehuda Brody and Noa Kinor and Amir Mor and Toshiro Tsukamoto and Spector, {David L.} and Singer, {Robert H.} and Yaron Shav-Tal",
year = "2010",
month = "5",
day = "15",
doi = "10.1242/jcs.062638",
language = "English (US)",
volume = "123",
pages = "1761--1774",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "10",

}

TY - JOUR

T1 - The life of an mRNA in space and time

AU - Ben-Ari, Ya'Ara

AU - Brody, Yehuda

AU - Kinor, Noa

AU - Mor, Amir

AU - Tsukamoto, Toshiro

AU - Spector, David L.

AU - Singer, Robert H.

AU - Shav-Tal, Yaron

PY - 2010/5/15

Y1 - 2010/5/15

N2 - Nuclear transcribed genes produce mRNA transcripts destined to travel from the site of transcription to the cytoplasm for protein translation. Certain transcripts can be further localized to specific cytoplasmic regions. We examined the life cycle of a transcribed β-actin mRNA throughout gene expression and localization, in a cell system that allows the in vivo detection of the gene locus, the transcribed mRNAs and the cytoplasmic β-actin protein that integrates into the actin cytoskeleton. Quantification showed that RNA polymerase II elongation progressed at a rate of 3.3 kb/minute and that transactivator binding to the promoter was transient (40 seconds), and demonstrated the unique spatial structure of the coding and non-coding regions of the integrated gene within the transcription site. The rates of gene induction were measured during interphase and after mitosis, demonstrating that daughter cells were not synchronized in respect to transcription initiation of the studied gene. Comparison of the spatial and temporal kinetics of nucleoplasmic and cytoplasmic mRNA transport showed that the β-actin-localization response initiates from the existing cytoplasmic mRNA pool and not from the newly synthesized transcripts arising after gene induction. It was also demonstrated that mechanisms of random movement were predominant in mediating the efficient translocation of mRNA in the eukaryotic cell.

AB - Nuclear transcribed genes produce mRNA transcripts destined to travel from the site of transcription to the cytoplasm for protein translation. Certain transcripts can be further localized to specific cytoplasmic regions. We examined the life cycle of a transcribed β-actin mRNA throughout gene expression and localization, in a cell system that allows the in vivo detection of the gene locus, the transcribed mRNAs and the cytoplasmic β-actin protein that integrates into the actin cytoskeleton. Quantification showed that RNA polymerase II elongation progressed at a rate of 3.3 kb/minute and that transactivator binding to the promoter was transient (40 seconds), and demonstrated the unique spatial structure of the coding and non-coding regions of the integrated gene within the transcription site. The rates of gene induction were measured during interphase and after mitosis, demonstrating that daughter cells were not synchronized in respect to transcription initiation of the studied gene. Comparison of the spatial and temporal kinetics of nucleoplasmic and cytoplasmic mRNA transport showed that the β-actin-localization response initiates from the existing cytoplasmic mRNA pool and not from the newly synthesized transcripts arising after gene induction. It was also demonstrated that mechanisms of random movement were predominant in mediating the efficient translocation of mRNA in the eukaryotic cell.

KW - Gene expression

KW - Live cell imaging

KW - mRNA dynamics

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=77952408391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952408391&partnerID=8YFLogxK

U2 - 10.1242/jcs.062638

DO - 10.1242/jcs.062638

M3 - Article

VL - 123

SP - 1761

EP - 1774

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 10

ER -