The L3 to L4 molt of Brugia malayi: Real time visualization by video microscopy

Manish Ramesh, Carol McGuiness, T. V. Rajan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Brugia malayi and other filarial parasites have been studied in great detail, especially in the context of human disease. In common with other nematodes, these organisms molt 4 times in their life cycles, but details of this process have not been described. We have recently developed an in vitro culture system that supports the L3 to L4 molt at high efficiency. This has permitted us to visualize, for the first time, details of this molt using real-time video microscopy. Molting is preceded by a phase of altered motility during which the larva exhibits contractile, coiling movements. The earliest evidence of ecdysis is a clearing at one end, more frequently caudal, caused by the larva retracting from that end. A cleavage develops in the cuticle near the head end, forming a rostral cap, which is continuous with the pharyngeal cuticle. Simultaneously, it retracts out of the cuticle using coiling and writhing movements. This process takes 5 to 10 min. Finally, it retracts out of the cap and extrudes the pharyngeal cuticle. Detachment of the pharyngeal cuticle is the final event in the process and continues up to an hour after the rest of the cuticle has been shed.

Original languageEnglish (US)
Pages (from-to)1028-1033
Number of pages6
JournalJournal of Parasitology
Volume91
Issue number5
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Brugia malayi
Molting
Video Microscopy
cuticle
molt
Larva
molting
visualization
microscopy
Life Cycle Stages
Parasites
Head
support systems
ecdysis
larvae
human diseases
in vitro culture
larva
life cycle (organisms)
Nematoda

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

The L3 to L4 molt of Brugia malayi : Real time visualization by video microscopy. / Ramesh, Manish; McGuiness, Carol; Rajan, T. V.

In: Journal of Parasitology, Vol. 91, No. 5, 10.2005, p. 1028-1033.

Research output: Contribution to journalArticle

Ramesh, Manish ; McGuiness, Carol ; Rajan, T. V. / The L3 to L4 molt of Brugia malayi : Real time visualization by video microscopy. In: Journal of Parasitology. 2005 ; Vol. 91, No. 5. pp. 1028-1033.
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