The isolation and purification of two anionic endothelial cell growth factors from human brain

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Abstract

Two anionic polypeptides which stimulate the proliferation of human umbilical vein endothelial cells have been isolated and purified to homogeneity from human brain using heparin affinity chromatography. The molecular weights of the polypeptides are 18,500 and 19,300; the isoelectric point for both polypeptides is at pH 5.2. The purified polypeptides differed in their ability to stimulate human endothelial cell growth. The half maximal activities observed for the 18.5 and 19.3 kilodalton polypeptides were at concentrations of 10.0 ng/ml and 0.9 ng/ml respectively.

Original languageEnglish (US)
Pages (from-to)262-268
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume124
Issue number1
DOIs
StatePublished - Oct 15 1984

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Endothelial Growth Factors
Endothelial cells
Cell growth
Purification
Brain
Intercellular Signaling Peptides and Proteins
Endothelial Cells
Peptides
Affinity chromatography
Human Umbilical Vein Endothelial Cells
Isoelectric Point
Affinity Chromatography
Heparin
Molecular Weight
Molecular weight
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AB - Two anionic polypeptides which stimulate the proliferation of human umbilical vein endothelial cells have been isolated and purified to homogeneity from human brain using heparin affinity chromatography. The molecular weights of the polypeptides are 18,500 and 19,300; the isoelectric point for both polypeptides is at pH 5.2. The purified polypeptides differed in their ability to stimulate human endothelial cell growth. The half maximal activities observed for the 18.5 and 19.3 kilodalton polypeptides were at concentrations of 10.0 ng/ml and 0.9 ng/ml respectively.

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