The interactions between cellular retinol-binding protein (CRBP-I) and retinal

A vibrational spectroscopic study

Larry Senak, Zhongmo Ju, Nao Noy, Robert Callender, Danny Manor

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Preresonance Raman difference spectra have been obtained for all-trans retinal in dilute CCl4 solution, complexed with cellular retinol-binding protein (CRBP-I) and retinol-binding protein (RBP). These spectra indicate that retinal is of a slightly more planar conformation within the binding pocket of CRBP-I than in solution or hydrophobically complexed with RBP. Compared to retinal in solution or bound to RBP, the conformation of the polyene tail of the retinal chromophore is perturbed from C8 through C11. This perturbation is probably due to the close proximity of the Lys40 in the CRBP-I binding pocket to the above-mentioned carbons. The C=O stretching vibration of bound retinal carbonyl has been found to shift from 1664 cm-1 solubilized in CCl4 to 1650 and 1645 cm-1 in RBP and CRBP-I, respectively, and significantly broadened in both cases. The frequency shift and broadening have been attributed to hydrogen bonding. These have been compared to calibrations of frequency shift (ΔνC=O) vs. ΔH and ΔG of all-trans retinal complexed with a series of phenol derivatives of incremental proton-donating ability as obtained by the relationship of van't Hoff. By this relationship, the binding enthalpy of the all-trans retinal carbonyl moiety bound to CRBP-I and RBP is -28.1 kJ/mol (-6.7 kcal/mol) and -23.5 kJ/mol (-5.6 kcal/ mol), respectively. The free energy of binding of the retinal carbonyl bound to CRBP-I and RBP has been determined to be -10.5 kJ/mol (-2.5 kcal/mol) and -7.2 kJ/mol (-1.7 kcal/mol), respectively. The hydrogen-bonded C=O moiety of retinal complexed with CRBP-I accounts for a substantial (25%) but not overriding amount of the binding energy of CRBP-I for retinal, and it also accounts for the protein's preference for binding retinol.

Original languageEnglish (US)
Pages (from-to)131-142
Number of pages12
JournalBiospectroscopy
Volume3
Issue number2
StatePublished - 1997
Externally publishedYes

Fingerprint

Cellular Retinol-Binding Proteins
Retinol-Binding Proteins
Protein Binding
Conformations
Polyenes
Carrier Proteins
Protein Conformation
Chromophores
Phenol
Binding energy
Hydrogen Bonding
Free energy
Vibration
Stretching
Protons
Hydrogen
Enthalpy
Hydrogen bonds
Calibration
Carbon

Keywords

  • Cellular retinol-binding protein
  • FTIR
  • Raman spectroscopy
  • Retinol-binding protein
  • Vibrational spectroscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The interactions between cellular retinol-binding protein (CRBP-I) and retinal : A vibrational spectroscopic study. / Senak, Larry; Ju, Zhongmo; Noy, Nao; Callender, Robert; Manor, Danny.

In: Biospectroscopy, Vol. 3, No. 2, 1997, p. 131-142.

Research output: Contribution to journalArticle

@article{de50d06da0b94c58b7f450c20e797680,
title = "The interactions between cellular retinol-binding protein (CRBP-I) and retinal: A vibrational spectroscopic study",
abstract = "Preresonance Raman difference spectra have been obtained for all-trans retinal in dilute CCl4 solution, complexed with cellular retinol-binding protein (CRBP-I) and retinol-binding protein (RBP). These spectra indicate that retinal is of a slightly more planar conformation within the binding pocket of CRBP-I than in solution or hydrophobically complexed with RBP. Compared to retinal in solution or bound to RBP, the conformation of the polyene tail of the retinal chromophore is perturbed from C8 through C11. This perturbation is probably due to the close proximity of the Lys40 in the CRBP-I binding pocket to the above-mentioned carbons. The C=O stretching vibration of bound retinal carbonyl has been found to shift from 1664 cm-1 solubilized in CCl4 to 1650 and 1645 cm-1 in RBP and CRBP-I, respectively, and significantly broadened in both cases. The frequency shift and broadening have been attributed to hydrogen bonding. These have been compared to calibrations of frequency shift (ΔνC=O) vs. ΔH and ΔG of all-trans retinal complexed with a series of phenol derivatives of incremental proton-donating ability as obtained by the relationship of van't Hoff. By this relationship, the binding enthalpy of the all-trans retinal carbonyl moiety bound to CRBP-I and RBP is -28.1 kJ/mol (-6.7 kcal/mol) and -23.5 kJ/mol (-5.6 kcal/ mol), respectively. The free energy of binding of the retinal carbonyl bound to CRBP-I and RBP has been determined to be -10.5 kJ/mol (-2.5 kcal/mol) and -7.2 kJ/mol (-1.7 kcal/mol), respectively. The hydrogen-bonded C=O moiety of retinal complexed with CRBP-I accounts for a substantial (25{\%}) but not overriding amount of the binding energy of CRBP-I for retinal, and it also accounts for the protein's preference for binding retinol.",
keywords = "Cellular retinol-binding protein, FTIR, Raman spectroscopy, Retinol-binding protein, Vibrational spectroscopy",
author = "Larry Senak and Zhongmo Ju and Nao Noy and Robert Callender and Danny Manor",
year = "1997",
language = "English (US)",
volume = "3",
pages = "131--142",
journal = "Biopolymers",
issn = "0006-3525",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - The interactions between cellular retinol-binding protein (CRBP-I) and retinal

T2 - A vibrational spectroscopic study

AU - Senak, Larry

AU - Ju, Zhongmo

AU - Noy, Nao

AU - Callender, Robert

AU - Manor, Danny

PY - 1997

Y1 - 1997

N2 - Preresonance Raman difference spectra have been obtained for all-trans retinal in dilute CCl4 solution, complexed with cellular retinol-binding protein (CRBP-I) and retinol-binding protein (RBP). These spectra indicate that retinal is of a slightly more planar conformation within the binding pocket of CRBP-I than in solution or hydrophobically complexed with RBP. Compared to retinal in solution or bound to RBP, the conformation of the polyene tail of the retinal chromophore is perturbed from C8 through C11. This perturbation is probably due to the close proximity of the Lys40 in the CRBP-I binding pocket to the above-mentioned carbons. The C=O stretching vibration of bound retinal carbonyl has been found to shift from 1664 cm-1 solubilized in CCl4 to 1650 and 1645 cm-1 in RBP and CRBP-I, respectively, and significantly broadened in both cases. The frequency shift and broadening have been attributed to hydrogen bonding. These have been compared to calibrations of frequency shift (ΔνC=O) vs. ΔH and ΔG of all-trans retinal complexed with a series of phenol derivatives of incremental proton-donating ability as obtained by the relationship of van't Hoff. By this relationship, the binding enthalpy of the all-trans retinal carbonyl moiety bound to CRBP-I and RBP is -28.1 kJ/mol (-6.7 kcal/mol) and -23.5 kJ/mol (-5.6 kcal/ mol), respectively. The free energy of binding of the retinal carbonyl bound to CRBP-I and RBP has been determined to be -10.5 kJ/mol (-2.5 kcal/mol) and -7.2 kJ/mol (-1.7 kcal/mol), respectively. The hydrogen-bonded C=O moiety of retinal complexed with CRBP-I accounts for a substantial (25%) but not overriding amount of the binding energy of CRBP-I for retinal, and it also accounts for the protein's preference for binding retinol.

AB - Preresonance Raman difference spectra have been obtained for all-trans retinal in dilute CCl4 solution, complexed with cellular retinol-binding protein (CRBP-I) and retinol-binding protein (RBP). These spectra indicate that retinal is of a slightly more planar conformation within the binding pocket of CRBP-I than in solution or hydrophobically complexed with RBP. Compared to retinal in solution or bound to RBP, the conformation of the polyene tail of the retinal chromophore is perturbed from C8 through C11. This perturbation is probably due to the close proximity of the Lys40 in the CRBP-I binding pocket to the above-mentioned carbons. The C=O stretching vibration of bound retinal carbonyl has been found to shift from 1664 cm-1 solubilized in CCl4 to 1650 and 1645 cm-1 in RBP and CRBP-I, respectively, and significantly broadened in both cases. The frequency shift and broadening have been attributed to hydrogen bonding. These have been compared to calibrations of frequency shift (ΔνC=O) vs. ΔH and ΔG of all-trans retinal complexed with a series of phenol derivatives of incremental proton-donating ability as obtained by the relationship of van't Hoff. By this relationship, the binding enthalpy of the all-trans retinal carbonyl moiety bound to CRBP-I and RBP is -28.1 kJ/mol (-6.7 kcal/mol) and -23.5 kJ/mol (-5.6 kcal/ mol), respectively. The free energy of binding of the retinal carbonyl bound to CRBP-I and RBP has been determined to be -10.5 kJ/mol (-2.5 kcal/mol) and -7.2 kJ/mol (-1.7 kcal/mol), respectively. The hydrogen-bonded C=O moiety of retinal complexed with CRBP-I accounts for a substantial (25%) but not overriding amount of the binding energy of CRBP-I for retinal, and it also accounts for the protein's preference for binding retinol.

KW - Cellular retinol-binding protein

KW - FTIR

KW - Raman spectroscopy

KW - Retinol-binding protein

KW - Vibrational spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=33746879907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746879907&partnerID=8YFLogxK

M3 - Article

VL - 3

SP - 131

EP - 142

JO - Biopolymers

JF - Biopolymers

SN - 0006-3525

IS - 2

ER -