The interaction of Pax5 (BSAP) with Daxx can result in transcriptional activation in B cells

Alexander Emelyanov, Cecilia R. Kovac, Manuel A. Sepulveda, Barbara K. Birshtein

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. Using a yeast two-hybrid assay to identify potential coregulators of Pax5, we identified Daxx, a protein that is highly conserved, ubiquitously expressed, and essential for embryonic mouse development. The interaction between Pax5 and Daxx involves the partial homeodomain of Pax5 and the C-terminal fragment of Daxx. A component of promyelocytic leukemia protein nuclear bodies, Daxx has been implicated in apoptosis and characterized as a transcriptional corepressor. Upon transient transfection assay of Daxx in B cells expressing endogenous Daxx and Pax5, we observed not only transcriptional corepression but also, unexpectedly, coactivation in M12.4.1 and A20 mouse B cell lines. Pax5 domains required for coactivation were identified using 293T cells. Coactivation apparently involves recruitment of the CREB binding protein (CBP), because we precipitated complexes containing Pax5, Daxx, and CBP in B cell lines. These data suggest that Daxx can affect Pax5's roles as an activator or repressor in B cells and describe a role for Daxx as a transcriptional coactivator.

Original languageEnglish (US)
Pages (from-to)11156-11164
Number of pages9
JournalJournal of Biological Chemistry
Volume277
Issue number13
DOIs
StatePublished - Mar 29 2002

Fingerprint

Transcriptional Activation
B-Lymphocytes
Chemical activation
Cells
CREB-Binding Protein
Assays
Cell Line
Co-Repressor Proteins
Two-Hybrid System Techniques
HEK293 Cells
Nuclear Proteins
Yeast
Embryonic Development
Transfection
Apoptosis
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

The interaction of Pax5 (BSAP) with Daxx can result in transcriptional activation in B cells. / Emelyanov, Alexander; Kovac, Cecilia R.; Sepulveda, Manuel A.; Birshtein, Barbara K.

In: Journal of Biological Chemistry, Vol. 277, No. 13, 29.03.2002, p. 11156-11164.

Research output: Contribution to journalArticle

Emelyanov, Alexander ; Kovac, Cecilia R. ; Sepulveda, Manuel A. ; Birshtein, Barbara K. / The interaction of Pax5 (BSAP) with Daxx can result in transcriptional activation in B cells. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 13. pp. 11156-11164.
@article{f18be042a32648daae40967505d2d587,
title = "The interaction of Pax5 (BSAP) with Daxx can result in transcriptional activation in B cells",
abstract = "Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. Using a yeast two-hybrid assay to identify potential coregulators of Pax5, we identified Daxx, a protein that is highly conserved, ubiquitously expressed, and essential for embryonic mouse development. The interaction between Pax5 and Daxx involves the partial homeodomain of Pax5 and the C-terminal fragment of Daxx. A component of promyelocytic leukemia protein nuclear bodies, Daxx has been implicated in apoptosis and characterized as a transcriptional corepressor. Upon transient transfection assay of Daxx in B cells expressing endogenous Daxx and Pax5, we observed not only transcriptional corepression but also, unexpectedly, coactivation in M12.4.1 and A20 mouse B cell lines. Pax5 domains required for coactivation were identified using 293T cells. Coactivation apparently involves recruitment of the CREB binding protein (CBP), because we precipitated complexes containing Pax5, Daxx, and CBP in B cell lines. These data suggest that Daxx can affect Pax5's roles as an activator or repressor in B cells and describe a role for Daxx as a transcriptional coactivator.",
author = "Alexander Emelyanov and Kovac, {Cecilia R.} and Sepulveda, {Manuel A.} and Birshtein, {Barbara K.}",
year = "2002",
month = "3",
day = "29",
doi = "10.1074/jbc.M111763200",
language = "English (US)",
volume = "277",
pages = "11156--11164",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "13",

}

TY - JOUR

T1 - The interaction of Pax5 (BSAP) with Daxx can result in transcriptional activation in B cells

AU - Emelyanov, Alexander

AU - Kovac, Cecilia R.

AU - Sepulveda, Manuel A.

AU - Birshtein, Barbara K.

PY - 2002/3/29

Y1 - 2002/3/29

N2 - Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. Using a yeast two-hybrid assay to identify potential coregulators of Pax5, we identified Daxx, a protein that is highly conserved, ubiquitously expressed, and essential for embryonic mouse development. The interaction between Pax5 and Daxx involves the partial homeodomain of Pax5 and the C-terminal fragment of Daxx. A component of promyelocytic leukemia protein nuclear bodies, Daxx has been implicated in apoptosis and characterized as a transcriptional corepressor. Upon transient transfection assay of Daxx in B cells expressing endogenous Daxx and Pax5, we observed not only transcriptional corepression but also, unexpectedly, coactivation in M12.4.1 and A20 mouse B cell lines. Pax5 domains required for coactivation were identified using 293T cells. Coactivation apparently involves recruitment of the CREB binding protein (CBP), because we precipitated complexes containing Pax5, Daxx, and CBP in B cell lines. These data suggest that Daxx can affect Pax5's roles as an activator or repressor in B cells and describe a role for Daxx as a transcriptional coactivator.

AB - Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. Using a yeast two-hybrid assay to identify potential coregulators of Pax5, we identified Daxx, a protein that is highly conserved, ubiquitously expressed, and essential for embryonic mouse development. The interaction between Pax5 and Daxx involves the partial homeodomain of Pax5 and the C-terminal fragment of Daxx. A component of promyelocytic leukemia protein nuclear bodies, Daxx has been implicated in apoptosis and characterized as a transcriptional corepressor. Upon transient transfection assay of Daxx in B cells expressing endogenous Daxx and Pax5, we observed not only transcriptional corepression but also, unexpectedly, coactivation in M12.4.1 and A20 mouse B cell lines. Pax5 domains required for coactivation were identified using 293T cells. Coactivation apparently involves recruitment of the CREB binding protein (CBP), because we precipitated complexes containing Pax5, Daxx, and CBP in B cell lines. These data suggest that Daxx can affect Pax5's roles as an activator or repressor in B cells and describe a role for Daxx as a transcriptional coactivator.

UR - http://www.scopus.com/inward/record.url?scp=0037192813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037192813&partnerID=8YFLogxK

U2 - 10.1074/jbc.M111763200

DO - 10.1074/jbc.M111763200

M3 - Article

C2 - 11799127

AN - SCOPUS:0037192813

VL - 277

SP - 11156

EP - 11164

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 13

ER -