The influence of insulin on circulating ghrelin

Daniel E. Flanagan, Mark L. Evans, Teresa P. Monsod, Frances Rife, Rubina A. Heptulla, William V. Tamborlane, Robert S. Sherwin

Research output: Contribution to journalArticle

302 Citations (Scopus)

Abstract

Ghrelin is a novel peptide that acts on the growth hormone (GH) secretagogue receptor in the pituitary and hypothalamus. It may function as a third physiological regulator of GH secretion, along with GH-releasing hormone and somatostatin. In addition to the action of ghrelin on the GH axis, it appears to have a role in the determination of energy homeostasis. Although feeding suppresses ghrelin production and fasting stimulates ghrelin release, the underlying mechanisms controlling this process remain unclear. The purpose of this study was to test the hypotheses, by use of a stepped hyperinsulinemic eu- hypo- hyperglycemic glucose clamp, that either hyperinsulinemia or hypoglycemia may influence ghrelin production. Having been stable in the period before the clamp, ghrelin levels rapidly fell in response to insulin infusion during euglycemia (baseline ghrelin 207 ± 12 vs. 169 ± 10 fmol/ml at t = 30 min, P < 0.001). Ghrelin remained suppressed during subsequent periods of hypoglycemia (mean glucose 53 ± 2 mg/dl) and hyperglycemia (mean glucose 163 ± 6 mg/dl). Despite suppression of ghrelin, GH showed a significant rise during hypoglycemia (baseline 4.1 ± 1.3 vs. 28.2 ± 3.9 μg/l at t = 120 min, P < 0.001). Our data suggest that insulin may suppress circulating ghrelin independently of glucose, although glucose may have an additional effect. We conclude that the GH response seen during hypoglycemia is not regulated by circulating ghrelin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume284
Issue number2 47-2
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

Ghrelin
Insulin
Hypoglycemia
Growth Hormone
Glucose
Clamping devices
Ghrelin Receptor
Growth Hormone-Releasing Hormone
Glucose Clamp Technique
Hyperinsulinism
Somatostatin
Hyperglycemia
Hypothalamus
Fasting
Homeostasis

Keywords

  • Glucose clamp
  • Growth hormone
  • Hypoglycemia
  • Hypothalamus
  • Somatostatin

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Flanagan, D. E., Evans, M. L., Monsod, T. P., Rife, F., Heptulla, R. A., Tamborlane, W. V., & Sherwin, R. S. (2003). The influence of insulin on circulating ghrelin. American Journal of Physiology - Endocrinology and Metabolism, 284(2 47-2).

The influence of insulin on circulating ghrelin. / Flanagan, Daniel E.; Evans, Mark L.; Monsod, Teresa P.; Rife, Frances; Heptulla, Rubina A.; Tamborlane, William V.; Sherwin, Robert S.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 284, No. 2 47-2, 01.02.2003.

Research output: Contribution to journalArticle

Flanagan, DE, Evans, ML, Monsod, TP, Rife, F, Heptulla, RA, Tamborlane, WV & Sherwin, RS 2003, 'The influence of insulin on circulating ghrelin', American Journal of Physiology - Endocrinology and Metabolism, vol. 284, no. 2 47-2.
Flanagan DE, Evans ML, Monsod TP, Rife F, Heptulla RA, Tamborlane WV et al. The influence of insulin on circulating ghrelin. American Journal of Physiology - Endocrinology and Metabolism. 2003 Feb 1;284(2 47-2).
Flanagan, Daniel E. ; Evans, Mark L. ; Monsod, Teresa P. ; Rife, Frances ; Heptulla, Rubina A. ; Tamborlane, William V. ; Sherwin, Robert S. / The influence of insulin on circulating ghrelin. In: American Journal of Physiology - Endocrinology and Metabolism. 2003 ; Vol. 284, No. 2 47-2.
@article{8760b3a53d494c9899be699c4c367127,
title = "The influence of insulin on circulating ghrelin",
abstract = "Ghrelin is a novel peptide that acts on the growth hormone (GH) secretagogue receptor in the pituitary and hypothalamus. It may function as a third physiological regulator of GH secretion, along with GH-releasing hormone and somatostatin. In addition to the action of ghrelin on the GH axis, it appears to have a role in the determination of energy homeostasis. Although feeding suppresses ghrelin production and fasting stimulates ghrelin release, the underlying mechanisms controlling this process remain unclear. The purpose of this study was to test the hypotheses, by use of a stepped hyperinsulinemic eu- hypo- hyperglycemic glucose clamp, that either hyperinsulinemia or hypoglycemia may influence ghrelin production. Having been stable in the period before the clamp, ghrelin levels rapidly fell in response to insulin infusion during euglycemia (baseline ghrelin 207 ± 12 vs. 169 ± 10 fmol/ml at t = 30 min, P < 0.001). Ghrelin remained suppressed during subsequent periods of hypoglycemia (mean glucose 53 ± 2 mg/dl) and hyperglycemia (mean glucose 163 ± 6 mg/dl). Despite suppression of ghrelin, GH showed a significant rise during hypoglycemia (baseline 4.1 ± 1.3 vs. 28.2 ± 3.9 μg/l at t = 120 min, P < 0.001). Our data suggest that insulin may suppress circulating ghrelin independently of glucose, although glucose may have an additional effect. We conclude that the GH response seen during hypoglycemia is not regulated by circulating ghrelin.",
keywords = "Glucose clamp, Growth hormone, Hypoglycemia, Hypothalamus, Somatostatin",
author = "Flanagan, {Daniel E.} and Evans, {Mark L.} and Monsod, {Teresa P.} and Frances Rife and Heptulla, {Rubina A.} and Tamborlane, {William V.} and Sherwin, {Robert S.}",
year = "2003",
month = "2",
day = "1",
language = "English (US)",
volume = "284",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2 47-2",

}

TY - JOUR

T1 - The influence of insulin on circulating ghrelin

AU - Flanagan, Daniel E.

AU - Evans, Mark L.

AU - Monsod, Teresa P.

AU - Rife, Frances

AU - Heptulla, Rubina A.

AU - Tamborlane, William V.

AU - Sherwin, Robert S.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Ghrelin is a novel peptide that acts on the growth hormone (GH) secretagogue receptor in the pituitary and hypothalamus. It may function as a third physiological regulator of GH secretion, along with GH-releasing hormone and somatostatin. In addition to the action of ghrelin on the GH axis, it appears to have a role in the determination of energy homeostasis. Although feeding suppresses ghrelin production and fasting stimulates ghrelin release, the underlying mechanisms controlling this process remain unclear. The purpose of this study was to test the hypotheses, by use of a stepped hyperinsulinemic eu- hypo- hyperglycemic glucose clamp, that either hyperinsulinemia or hypoglycemia may influence ghrelin production. Having been stable in the period before the clamp, ghrelin levels rapidly fell in response to insulin infusion during euglycemia (baseline ghrelin 207 ± 12 vs. 169 ± 10 fmol/ml at t = 30 min, P < 0.001). Ghrelin remained suppressed during subsequent periods of hypoglycemia (mean glucose 53 ± 2 mg/dl) and hyperglycemia (mean glucose 163 ± 6 mg/dl). Despite suppression of ghrelin, GH showed a significant rise during hypoglycemia (baseline 4.1 ± 1.3 vs. 28.2 ± 3.9 μg/l at t = 120 min, P < 0.001). Our data suggest that insulin may suppress circulating ghrelin independently of glucose, although glucose may have an additional effect. We conclude that the GH response seen during hypoglycemia is not regulated by circulating ghrelin.

AB - Ghrelin is a novel peptide that acts on the growth hormone (GH) secretagogue receptor in the pituitary and hypothalamus. It may function as a third physiological regulator of GH secretion, along with GH-releasing hormone and somatostatin. In addition to the action of ghrelin on the GH axis, it appears to have a role in the determination of energy homeostasis. Although feeding suppresses ghrelin production and fasting stimulates ghrelin release, the underlying mechanisms controlling this process remain unclear. The purpose of this study was to test the hypotheses, by use of a stepped hyperinsulinemic eu- hypo- hyperglycemic glucose clamp, that either hyperinsulinemia or hypoglycemia may influence ghrelin production. Having been stable in the period before the clamp, ghrelin levels rapidly fell in response to insulin infusion during euglycemia (baseline ghrelin 207 ± 12 vs. 169 ± 10 fmol/ml at t = 30 min, P < 0.001). Ghrelin remained suppressed during subsequent periods of hypoglycemia (mean glucose 53 ± 2 mg/dl) and hyperglycemia (mean glucose 163 ± 6 mg/dl). Despite suppression of ghrelin, GH showed a significant rise during hypoglycemia (baseline 4.1 ± 1.3 vs. 28.2 ± 3.9 μg/l at t = 120 min, P < 0.001). Our data suggest that insulin may suppress circulating ghrelin independently of glucose, although glucose may have an additional effect. We conclude that the GH response seen during hypoglycemia is not regulated by circulating ghrelin.

KW - Glucose clamp

KW - Growth hormone

KW - Hypoglycemia

KW - Hypothalamus

KW - Somatostatin

UR - http://www.scopus.com/inward/record.url?scp=0037302210&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037302210&partnerID=8YFLogxK

M3 - Article

C2 - 12531744

AN - SCOPUS:0037302210

VL - 284

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 2 47-2

ER -