The in vitro effects of Trolox on methylmercury-induced neurotoxicity

Parvinder Kaur, Lars Evje, Michael Aschner, Tore Syversen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Methylmercury (MeHg), an environmental toxicant primarily found in fish and seafood poses a dilemma to both consumers and regulatory authorities given the nutritional benefits of fish consumption vs. possible adverse neurological damage caused by MeHg. The present study addresses whether supplementation with 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), alters the neuro-oxidative effects of MeHg in C6-glioma and B35-neuronal cell lines. As indicators of cytotoxicity, reduced glutathione (GSH), reactive oxygen species (ROS) and mitochondrial activity (MTT) were measured. The cellular mercury (Hg) content was measured with high resolution-inductively coupled plasma mass spectrometry (HR-ICPMS). The amount of MeHg-induced ROS was significantly reduced (. p<. 0.05) after treatment with 50. μM Trolox in C6 glial cell line. However, treatment with Trolox did not induce any significant increase in GSH levels or MTT activity in either of the cell lines. In addition, treatment with Trolox did not induce any significant changes in intracellular MeHg levels. The MeHg and Trolox treated C6 glial cell line differed significantly (. p<. 0.05) from the B35 cell line for MTT, ROS and GSH activity. These findings provide experimental evidence that preincubation with Trolox prevents MeHg-induced ROS generation in C6 glial cell line by quenching of free radicals and not by changes in intracellular GSH or MeHg content.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalToxicology
Volume276
Issue number1
DOIs
StatePublished - Sep 2010
Externally publishedYes

Fingerprint

Cell Line
Reactive Oxygen Species
Neuroglia
Cells
Fish
Fishes
Inductively coupled plasma mass spectrometry
Seafood
Cytotoxicity
Carboxylic Acids
Mercury
Glioma
Free Radicals
Glutathione
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
In Vitro Techniques
Quenching
Mass Spectrometry

Keywords

  • Cell cultures
  • In vitro
  • Neurotoxicity
  • Reactive oxygen species
  • Reduced glutathione
  • Trolox

ASJC Scopus subject areas

  • Toxicology

Cite this

The in vitro effects of Trolox on methylmercury-induced neurotoxicity. / Kaur, Parvinder; Evje, Lars; Aschner, Michael; Syversen, Tore.

In: Toxicology, Vol. 276, No. 1, 09.2010, p. 73-78.

Research output: Contribution to journalArticle

Kaur, Parvinder ; Evje, Lars ; Aschner, Michael ; Syversen, Tore. / The in vitro effects of Trolox on methylmercury-induced neurotoxicity. In: Toxicology. 2010 ; Vol. 276, No. 1. pp. 73-78.
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