TY - JOUR
T1 - The impact of nucleosome positioning on the organization of replication origins in eukaryotes
AU - Yin, Shanye
AU - Deng, Wenjun
AU - Hu, Landian
AU - Kong, Xiangyin
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (No. 30871356), the National High Technology Research and Development Program of China (2006AA02Z330), the National Basic Research Program of China (2004CB518603), and the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX1-YW-R-74).
PY - 2009/7/31
Y1 - 2009/7/31
N2 - The distribution of DNA replication origins (ORIs) on eukaryotic chromosomes is nonrandom, but the reasons behind this are not well understood. Previous studies have suggested a prominent role of transcriptional activity in determining the ORI organization. Here, we identify nucleosome occupancy as a likely candidate to set up ORI distribution. Combining genome-wide data on nucleosome positioning and ORI organization in yeast and humans, we demonstrate that open chromatin domains, characterized by nucleosome depletion, are preferentially permissive for replication. However, contrary to priori claims, the impact of transcriptional activity is considerably weaker than previously proposed and could partly be explained by our nucleosome exclusion model. We propose that the ORI organization imposed by nucleosome positioning is phylogenetically widespread in eukaryotes.
AB - The distribution of DNA replication origins (ORIs) on eukaryotic chromosomes is nonrandom, but the reasons behind this are not well understood. Previous studies have suggested a prominent role of transcriptional activity in determining the ORI organization. Here, we identify nucleosome occupancy as a likely candidate to set up ORI distribution. Combining genome-wide data on nucleosome positioning and ORI organization in yeast and humans, we demonstrate that open chromatin domains, characterized by nucleosome depletion, are preferentially permissive for replication. However, contrary to priori claims, the impact of transcriptional activity is considerably weaker than previously proposed and could partly be explained by our nucleosome exclusion model. We propose that the ORI organization imposed by nucleosome positioning is phylogenetically widespread in eukaryotes.
KW - DNA replication origin (ORI)
KW - Nucleosome positioning
KW - Transcriptional activity
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U2 - 10.1016/j.bbrc.2009.05.072
DO - 10.1016/j.bbrc.2009.05.072
M3 - Article
C2 - 19463783
AN - SCOPUS:67349272585
SN - 0006-291X
VL - 385
SP - 363
EP - 368
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -