The identification of nucleoside triphosphate ends on RNA formed in the RNA polymerase reaction

Umadas Maitra; Abraham Novogrodsky; David Baltimore; Jerard Hurwitz

doi:10.1016/0006-291X(65)90857-0

The identification of nucleoside triphosphate ends on RNA formed in the RNA polymerase reaction

Umadas Maitra, Abraham Novogrodsky, David Baltimore, Jerard Hurwitz

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

RNA polymerase reactions primed with a variety of different DNA preparations (T2, thymus, denatured thymus and dAT copolymer) lead to the incorporation of ³²P from β-γ ³²P-labeled ATP into an acid-insoluble form. Evidence has been presented which indicates that the triphosphate group is present at the end of RNA chains.

Original language	English (US)
Pages (from-to)	801-811
Number of pages	11
Journal	Biochemical and Biophysical Research Communications
Volume	18
Issue number	5-6
DOIs	https://doi.org/10.1016/0006-291X(65)90857-0
State	Published - Mar 12 1965

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The identification of nucleoside triphosphate ends on RNA formed in the RNA polymerase reaction",

abstract = "RNA polymerase reactions primed with a variety of different DNA preparations (T2, thymus, denatured thymus and dAT copolymer) lead to the incorporation of 32P from β-γ 32P-labeled ATP into an acid-insoluble form. Evidence has been presented which indicates that the triphosphate group is present at the end of RNA chains.",

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AU - Hurwitz, Jerard

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AB - RNA polymerase reactions primed with a variety of different DNA preparations (T2, thymus, denatured thymus and dAT copolymer) lead to the incorporation of 32P from β-γ 32P-labeled ATP into an acid-insoluble form. Evidence has been presented which indicates that the triphosphate group is present at the end of RNA chains.

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